Copper treatment activates mitogen-activated protein kinase signalling in rice

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

It is well known that mitogen-activated protein kinase (MAPK) pathways are modules involved in the transduction of extracellular signals to intracellular targets in all eukaryotes. In plants, it has been shown that MAPKs play a role in the signalling of biotic and abiotic stresses. To characterize signalling pathways involved in heavy metal-induced stress responses, we examine whether plant MAPKs are also involved in this process. The analyses of mRNA levels of OsMAPK genes have shown that only OsMAPK2 mRNA transcripts increased within 12h upon CuCl2 treatment in suspension cells and roots. An in-gel kinase assay revealed that three protein kinases, approximate 42, 50, and 64-kDa, were activated by CuCl2 treatments. The approximate 42-kDa protein kinase displayed MAPK properties. Antioxidant, GSH, prevented copper-induced kinase activity. Furthermore, we found that rice roots underwent a rapid cell death upon this copper treatment. The copper-induced cell death of rice roots was partially blocked by MAPK kinase inhibitor, PD98059. These results suggest that the MAPK cascades may function in the plant heavy metal induced-signalling pathway.

Original languageEnglish
Pages (from-to)392-399
Number of pages8
JournalPhysiologia plantarum
Volume119
Issue number3
DOIs
Publication statusPublished - 2003 Nov 1

Fingerprint

Mitogen-Activated Protein Kinases
mitogen-activated protein kinase
Copper
mitogen-activated protein kinase kinase
copper
Heavy Metals
rice
protein kinases
Protein Kinases
cell death
phosphotransferases (kinases)
heavy metals
Cell Death
Phosphotransferases
Messenger RNA
Mitogen-Activated Protein Kinase Kinases
biotic stress
Eukaryota
cell suspension culture
abiotic stress

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

@article{ca7fa4c524b547ef9c38769043b5bb66,
title = "Copper treatment activates mitogen-activated protein kinase signalling in rice",
abstract = "It is well known that mitogen-activated protein kinase (MAPK) pathways are modules involved in the transduction of extracellular signals to intracellular targets in all eukaryotes. In plants, it has been shown that MAPKs play a role in the signalling of biotic and abiotic stresses. To characterize signalling pathways involved in heavy metal-induced stress responses, we examine whether plant MAPKs are also involved in this process. The analyses of mRNA levels of OsMAPK genes have shown that only OsMAPK2 mRNA transcripts increased within 12h upon CuCl2 treatment in suspension cells and roots. An in-gel kinase assay revealed that three protein kinases, approximate 42, 50, and 64-kDa, were activated by CuCl2 treatments. The approximate 42-kDa protein kinase displayed MAPK properties. Antioxidant, GSH, prevented copper-induced kinase activity. Furthermore, we found that rice roots underwent a rapid cell death upon this copper treatment. The copper-induced cell death of rice roots was partially blocked by MAPK kinase inhibitor, PD98059. These results suggest that the MAPK cascades may function in the plant heavy metal induced-signalling pathway.",
author = "Yeh, {Chuan Ming} and Hung, {Wan Chi} and Huang, {Hao Jen}",
year = "2003",
month = "11",
day = "1",
doi = "10.1034/j.1399-3054.2003.00191.x",
language = "English",
volume = "119",
pages = "392--399",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
number = "3",

}

Copper treatment activates mitogen-activated protein kinase signalling in rice. / Yeh, Chuan Ming; Hung, Wan Chi; Huang, Hao Jen.

In: Physiologia plantarum, Vol. 119, No. 3, 01.11.2003, p. 392-399.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Copper treatment activates mitogen-activated protein kinase signalling in rice

AU - Yeh, Chuan Ming

AU - Hung, Wan Chi

AU - Huang, Hao Jen

PY - 2003/11/1

Y1 - 2003/11/1

N2 - It is well known that mitogen-activated protein kinase (MAPK) pathways are modules involved in the transduction of extracellular signals to intracellular targets in all eukaryotes. In plants, it has been shown that MAPKs play a role in the signalling of biotic and abiotic stresses. To characterize signalling pathways involved in heavy metal-induced stress responses, we examine whether plant MAPKs are also involved in this process. The analyses of mRNA levels of OsMAPK genes have shown that only OsMAPK2 mRNA transcripts increased within 12h upon CuCl2 treatment in suspension cells and roots. An in-gel kinase assay revealed that three protein kinases, approximate 42, 50, and 64-kDa, were activated by CuCl2 treatments. The approximate 42-kDa protein kinase displayed MAPK properties. Antioxidant, GSH, prevented copper-induced kinase activity. Furthermore, we found that rice roots underwent a rapid cell death upon this copper treatment. The copper-induced cell death of rice roots was partially blocked by MAPK kinase inhibitor, PD98059. These results suggest that the MAPK cascades may function in the plant heavy metal induced-signalling pathway.

AB - It is well known that mitogen-activated protein kinase (MAPK) pathways are modules involved in the transduction of extracellular signals to intracellular targets in all eukaryotes. In plants, it has been shown that MAPKs play a role in the signalling of biotic and abiotic stresses. To characterize signalling pathways involved in heavy metal-induced stress responses, we examine whether plant MAPKs are also involved in this process. The analyses of mRNA levels of OsMAPK genes have shown that only OsMAPK2 mRNA transcripts increased within 12h upon CuCl2 treatment in suspension cells and roots. An in-gel kinase assay revealed that three protein kinases, approximate 42, 50, and 64-kDa, were activated by CuCl2 treatments. The approximate 42-kDa protein kinase displayed MAPK properties. Antioxidant, GSH, prevented copper-induced kinase activity. Furthermore, we found that rice roots underwent a rapid cell death upon this copper treatment. The copper-induced cell death of rice roots was partially blocked by MAPK kinase inhibitor, PD98059. These results suggest that the MAPK cascades may function in the plant heavy metal induced-signalling pathway.

UR - http://www.scopus.com/inward/record.url?scp=0242309076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242309076&partnerID=8YFLogxK

U2 - 10.1034/j.1399-3054.2003.00191.x

DO - 10.1034/j.1399-3054.2003.00191.x

M3 - Article

AN - SCOPUS:0242309076

VL - 119

SP - 392

EP - 399

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 3

ER -