Copy number variant hotspots in Han Taiwanese population induced pluripotent stem cell lines - Lessons from establishing the Taiwan human disease iPSC Consortium Bank

Ching Ying Huang, Ling Hui Li, Wan Tseng Hsu, Yu Che Cheng, Martin W. Nicholson, Chun Lin Liu, Chien Yu Ting, Hui Wen Ko, Shih Han Syu, Cheng Hao Wen, Zhuge Yan, Hsiang Po Huang, Hong Lin Su, Po Min Chiang, Chia Ning Shen, Hsin Fu Chen, B. Lin Ju Yen, Huai En Lu, Shiaw Min Hwang, Shih Hwa ChiouHong Nerng Ho, Jer Yuarn Wu, Timothy J. Kamp, Joseph C. Wu, Patrick C.H. Hsieh

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The Taiwan Human Disease iPSC Service Consortium was established to accelerate Taiwan's growing stem cell research initiatives and provide a platform for researchers interested in utilizing induced pluripotent stem cell (iPSC) technology. The consortium has generated and characterized 83 iPSC lines: 11 normal and 72 disease iPSC lines covering 21 different diseases, several of which are of high incidence in Taiwan. Whether there are any reprogramming-induced recurrent copy number variant (CNV) hotspots in iPSCs is still largely unknown. Methods: We performed genome-wide copy number variant screening of 83 Han Taiwanese iPSC lines and compared them with 1093 control subjects using an Affymetrix genome-wide human SNP array. Results: In the iPSCs, we identified ten specific CNV loci and seven "polymorphic"CNV regions that are associated with the reprogramming process. Additionally, we established several differentiation protocols for our iPSC lines. We demonstrated that our iPSC-derived cardiomyocytes respond to pharmacological agents and were successfully engrafted into the mouse myocardium demonstrating their potential application in cell therapy. Conclusions: The CNV hotspots induced by cell reprogramming have successfully been identified in the current study. This finding may be used as a reference index for evaluating iPSC quality for future clinical applications. Our aim was to establish a national iPSC resource center generating iPSCs, made available to researchers, to benefit the stem cell community in Taiwan and throughout the world.

Original languageEnglish
Article number92
JournalJournal of biomedical science
Volume27
Issue number1
DOIs
Publication statusPublished - 2020 Sep 4

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

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