Correlation between serum levels of anti-endothelial cell autoantigen and anti-dengue virus nonstructural protein 1 antibodies in dengue patients

Hsien Jen Cheng, Yueh Hsia Luo, Shu Wen Wan, Chiou Feng Lin, Shan-Tair Wang, Nguyen Thanh Hung, Ching-Chuan Liu, Tzong-Shiann Ho, Hsiao-Sheng Liu, Trai-Ming Yeh, Yee-Shin Lin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have previously shown that anti-dengue virus nonstructural protein 1 (anti-DENV NS1) antibodies cross-react with endothelial cells, and several autoantigens have been identified. This study shows that the antibody levels against these self-proteins are higher in sera from patients with dengue hemorrhagic fever (DHF) than those in control sera. Anti-protein disulfide isomerase (PDI) and anti-heat shock protein 60 (anti-HSP60) IgM levels correlated with both anti-endothelial cells and anti-DENV NS1 IgM titers. A cross-reactive epitope on the NS1 amino acid residues 311-330 (P311-330) had been predicted. We further found that there were higher IgM and IgG levels against P311-330 in DHF patients' sera than those in the control sera. In addition, correlations were observed between anti-PDI with anti-P311-330 IgM and IgG levels, respectively. Therefore, our results indicate that DENV NS1 P311-330 is a major epitope for cross-reactive antibodies to PDI on the endothelial cell surface, which may play an important role in DENV infection-induced autoimmunity.

Original languageEnglish
Pages (from-to)989-995
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume92
Issue number5
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

Dengue Virus
Dengue
Autoantigens
Protein Disulfide-Isomerases
Immunoglobulin M
Endothelial Cells
Severe Dengue
Antibodies
Serum
Epitopes
Proteins
Immunoglobulin G
Chaperonin 60
Autoimmunity
Amino Acids
Infection

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

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title = "Correlation between serum levels of anti-endothelial cell autoantigen and anti-dengue virus nonstructural protein 1 antibodies in dengue patients",
abstract = "We have previously shown that anti-dengue virus nonstructural protein 1 (anti-DENV NS1) antibodies cross-react with endothelial cells, and several autoantigens have been identified. This study shows that the antibody levels against these self-proteins are higher in sera from patients with dengue hemorrhagic fever (DHF) than those in control sera. Anti-protein disulfide isomerase (PDI) and anti-heat shock protein 60 (anti-HSP60) IgM levels correlated with both anti-endothelial cells and anti-DENV NS1 IgM titers. A cross-reactive epitope on the NS1 amino acid residues 311-330 (P311-330) had been predicted. We further found that there were higher IgM and IgG levels against P311-330 in DHF patients' sera than those in the control sera. In addition, correlations were observed between anti-PDI with anti-P311-330 IgM and IgG levels, respectively. Therefore, our results indicate that DENV NS1 P311-330 is a major epitope for cross-reactive antibodies to PDI on the endothelial cell surface, which may play an important role in DENV infection-induced autoimmunity.",
author = "Cheng, {Hsien Jen} and Luo, {Yueh Hsia} and Wan, {Shu Wen} and Lin, {Chiou Feng} and Shan-Tair Wang and Hung, {Nguyen Thanh} and Ching-Chuan Liu and Tzong-Shiann Ho and Hsiao-Sheng Liu and Trai-Ming Yeh and Yee-Shin Lin",
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Correlation between serum levels of anti-endothelial cell autoantigen and anti-dengue virus nonstructural protein 1 antibodies in dengue patients. / Cheng, Hsien Jen; Luo, Yueh Hsia; Wan, Shu Wen; Lin, Chiou Feng; Wang, Shan-Tair; Hung, Nguyen Thanh; Liu, Ching-Chuan; Ho, Tzong-Shiann; Liu, Hsiao-Sheng; Yeh, Trai-Ming; Lin, Yee-Shin.

In: American Journal of Tropical Medicine and Hygiene, Vol. 92, No. 5, 01.05.2015, p. 989-995.

Research output: Contribution to journalArticle

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AU - Liu, Ching-Chuan

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AU - Yeh, Trai-Ming

AU - Lin, Yee-Shin

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