Abstract
Muscle strain is still awanting a noninvasive quantitatively diagnosis tool. High frequency ultrasound (HFU) improves image resolution for monitoring changes of tissue structures, but the biomechanical factors may influence ultrasonography during injury detection. We aim to illustrate the ultrasonic parameters to present the histological damage of overstretched muscle with the consideration of biomechanical factors. Gastrocnemius muscles from mice were assembled and ex vivo passive stretching was performed before or after injury. After injury, the muscle significantly decreased mechanical strength. Ultrasonic images were obtained by HFU at different deformations to scan in cross and longitudinal orientations of muscle. The ultrasonography was quantified by echogenicity and Nakagami parameters (NP) for structural evaluation and correlated with histological results. The injured muscle at its original length exhibited decreased echogenicity and NP from HFU images. Cross-sectional ultrasonography revealed a loss of correlation between NP and passive muscle stretching that suggested a special scatterer pattern in the cross section of injured muscle. The independence of NP during passive stretching of injured muscle was confirmed by histological findings in ruptured collagen fibers, decreased muscle density, and increased intermuscular fiber space. Thus, HFU analysis of NP in cross section represents muscle injury that may benefit the clinical diagnosis.
| Original language | English |
|---|---|
| Article number | 6893712 |
| Journal | BioMed research international |
| Volume | 2016 |
| DOIs | |
| Publication status | Published - 2016 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
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Cross-Sectional Nakagami Images in Passive Stretches Reveal Damage of Injured Muscles. / Lin, Shih Ping; Lin, Yi Hsun; Fan, Shih Chen; Huang, Bu Miin; Lin, Wei Yin; Wang, Shyh Hau; Shung, K. Kirk; Su, Fong Chin; Wu, Chia Ching.
In: BioMed research international, Vol. 2016, 6893712, 01.01.2016.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cross-Sectional Nakagami Images in Passive Stretches Reveal Damage of Injured Muscles
AU - Lin, Shih Ping
AU - Lin, Yi Hsun
AU - Fan, Shih Chen
AU - Huang, Bu Miin
AU - Lin, Wei Yin
AU - Wang, Shyh Hau
AU - Shung, K. Kirk
AU - Su, Fong Chin
AU - Wu, Chia Ching
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Muscle strain is still awanting a noninvasive quantitatively diagnosis tool. High frequency ultrasound (HFU) improves image resolution for monitoring changes of tissue structures, but the biomechanical factors may influence ultrasonography during injury detection. We aim to illustrate the ultrasonic parameters to present the histological damage of overstretched muscle with the consideration of biomechanical factors. Gastrocnemius muscles from mice were assembled and ex vivo passive stretching was performed before or after injury. After injury, the muscle significantly decreased mechanical strength. Ultrasonic images were obtained by HFU at different deformations to scan in cross and longitudinal orientations of muscle. The ultrasonography was quantified by echogenicity and Nakagami parameters (NP) for structural evaluation and correlated with histological results. The injured muscle at its original length exhibited decreased echogenicity and NP from HFU images. Cross-sectional ultrasonography revealed a loss of correlation between NP and passive muscle stretching that suggested a special scatterer pattern in the cross section of injured muscle. The independence of NP during passive stretching of injured muscle was confirmed by histological findings in ruptured collagen fibers, decreased muscle density, and increased intermuscular fiber space. Thus, HFU analysis of NP in cross section represents muscle injury that may benefit the clinical diagnosis.
AB - Muscle strain is still awanting a noninvasive quantitatively diagnosis tool. High frequency ultrasound (HFU) improves image resolution for monitoring changes of tissue structures, but the biomechanical factors may influence ultrasonography during injury detection. We aim to illustrate the ultrasonic parameters to present the histological damage of overstretched muscle with the consideration of biomechanical factors. Gastrocnemius muscles from mice were assembled and ex vivo passive stretching was performed before or after injury. After injury, the muscle significantly decreased mechanical strength. Ultrasonic images were obtained by HFU at different deformations to scan in cross and longitudinal orientations of muscle. The ultrasonography was quantified by echogenicity and Nakagami parameters (NP) for structural evaluation and correlated with histological results. The injured muscle at its original length exhibited decreased echogenicity and NP from HFU images. Cross-sectional ultrasonography revealed a loss of correlation between NP and passive muscle stretching that suggested a special scatterer pattern in the cross section of injured muscle. The independence of NP during passive stretching of injured muscle was confirmed by histological findings in ruptured collagen fibers, decreased muscle density, and increased intermuscular fiber space. Thus, HFU analysis of NP in cross section represents muscle injury that may benefit the clinical diagnosis.
UR - http://www.scopus.com/inward/record.url?scp=84955580490&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84955580490&partnerID=8YFLogxK
U2 - 10.1155/2016/6893712
DO - 10.1155/2016/6893712
M3 - Article
C2 - 27034946
AN - SCOPUS:84955580490
VL - 2016
JO - BioMed Research International
JF - BioMed Research International
SN - 2314-6133
M1 - 6893712
ER -