TY - JOUR
T1 - Crosslinked CNBr-activated hyaluronan-collagen matrices
T2 - Effects on fibroblast contraction
AU - Huang-Lee, Lynn L.H.
AU - Nimni, Marcel E.
PY - 1994/3
Y1 - 1994/3
N2 - Previously we demonstrated that the contraction of collagen fibrillar matrices (CFM) by fibroblasts was significantly reduced when high concentrations (> 1 mg/ml) of hyaluronan were present in the media. This highlights the prominent role of hyaluronan in preventing scar formation during fetal wound healing. In the present study, we covalently crosslinked different concentrations of CNBr-activated hyaluronan to CFM and investigated the ability of fibroblasts to contract these matrices. Matrices are not contracted when optimal concentrations of CNBr-activated hyaluronan are crosslinked to collagen. Biochemical and cytological analysis of these matrice suggests that hyaluronan strengthens the collagen fibrils and blocks direct communication between fibroblasts and the collagen fibrils. Based on current evidence we are able to propose two molecular crosslinking models, multiple point attachment and simple point attachment between CNBr-activated hyaluronan and collagen molecules, hypotheses supported by the susceptibility of these matrice to digestion by collagenase.
AB - Previously we demonstrated that the contraction of collagen fibrillar matrices (CFM) by fibroblasts was significantly reduced when high concentrations (> 1 mg/ml) of hyaluronan were present in the media. This highlights the prominent role of hyaluronan in preventing scar formation during fetal wound healing. In the present study, we covalently crosslinked different concentrations of CNBr-activated hyaluronan to CFM and investigated the ability of fibroblasts to contract these matrices. Matrices are not contracted when optimal concentrations of CNBr-activated hyaluronan are crosslinked to collagen. Biochemical and cytological analysis of these matrice suggests that hyaluronan strengthens the collagen fibrils and blocks direct communication between fibroblasts and the collagen fibrils. Based on current evidence we are able to propose two molecular crosslinking models, multiple point attachment and simple point attachment between CNBr-activated hyaluronan and collagen molecules, hypotheses supported by the susceptibility of these matrice to digestion by collagenase.
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U2 - 10.1016/0945-053X(94)90004-3
DO - 10.1016/0945-053X(94)90004-3
M3 - Article
C2 - 8061927
AN - SCOPUS:0028347854
SN - 0945-053X
VL - 14
SP - 147
EP - 157
JO - Matrix Biology
JF - Matrix Biology
IS - 2
ER -