Crosslinked CNBr-activated hyaluronan-collagen matrices: Effects on fibroblast contraction

Lynn L.H. Huang-Lee, Marcel E. Nimni

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Previously we demonstrated that the contraction of collagen fibrillar matrices (CFM) by fibroblasts was significantly reduced when high concentrations (> 1 mg/ml) of hyaluronan were present in the media. This highlights the prominent role of hyaluronan in preventing scar formation during fetal wound healing. In the present study, we covalently crosslinked different concentrations of CNBr-activated hyaluronan to CFM and investigated the ability of fibroblasts to contract these matrices. Matrices are not contracted when optimal concentrations of CNBr-activated hyaluronan are crosslinked to collagen. Biochemical and cytological analysis of these matrice suggests that hyaluronan strengthens the collagen fibrils and blocks direct communication between fibroblasts and the collagen fibrils. Based on current evidence we are able to propose two molecular crosslinking models, multiple point attachment and simple point attachment between CNBr-activated hyaluronan and collagen molecules, hypotheses supported by the susceptibility of these matrice to digestion by collagenase.

Original languageEnglish
Pages (from-to)147-157
Number of pages11
JournalMatrix Biology
Volume14
Issue number2
DOIs
Publication statusPublished - 1994 Mar

All Science Journal Classification (ASJC) codes

  • Molecular Biology

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