TY - JOUR
T1 - Crosslinked on novel nanofibers with thermophilic carbonic anhydrase for carbon dioxide sequestration
AU - Effendi, Sefli Sri Wahyu
AU - Chiu, Chen Yaw
AU - Chang, Yu Kaung
AU - Ng, I. Son
N1 - Funding Information:
The authors are grateful to the financial support for this study provided by the Ministry of Science and Technology ( MOST 108-2621-M-006-015 , MOST 108-2221-E-006-004-MY3 and MOST 108-2218-E-006-006 ) in Taiwan.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - The recombinant Sulfurihydrogenibium yellowstonense carbonic anhydrase (SyCA) was covalently bonded on novel polyacrylonitrile (PAN) and polyethylene terephthalate (PET) nanofibers (PAN-PET-PAN donated as AEA) that was first fabricated by electrospinning. The resulting composite materials further crosslinked by the glutaraldehyde, which significantly increased thermostability up to 89.8% and 18.0% after heating at 60 °C for 1 h for immobilized crude and pure SyCA, respectively. After four repetitive attempts in the demonstration of CO2 sequestration, immobilized crude and pure SyCA on AEA also effectively improved the total CaCO3 yields to be 5.8 folds and 2.2 folds compared to free enzyme. Furthermore, the endurance of immobilized crude was investigated on flue gasses, which was retained its activity up to 57% on 50 mM NOx and 61% on 50 mM SOx presence. This is the first report of immobilized thermophilic SyCA on a novel nanofiber at the reusability, durability, sequestration of carbon dioxide, tolerant to sulfur oxides (SOx) and nitrogen oxides (NOx) toxic gases and to prevent air pollution.
AB - The recombinant Sulfurihydrogenibium yellowstonense carbonic anhydrase (SyCA) was covalently bonded on novel polyacrylonitrile (PAN) and polyethylene terephthalate (PET) nanofibers (PAN-PET-PAN donated as AEA) that was first fabricated by electrospinning. The resulting composite materials further crosslinked by the glutaraldehyde, which significantly increased thermostability up to 89.8% and 18.0% after heating at 60 °C for 1 h for immobilized crude and pure SyCA, respectively. After four repetitive attempts in the demonstration of CO2 sequestration, immobilized crude and pure SyCA on AEA also effectively improved the total CaCO3 yields to be 5.8 folds and 2.2 folds compared to free enzyme. Furthermore, the endurance of immobilized crude was investigated on flue gasses, which was retained its activity up to 57% on 50 mM NOx and 61% on 50 mM SOx presence. This is the first report of immobilized thermophilic SyCA on a novel nanofiber at the reusability, durability, sequestration of carbon dioxide, tolerant to sulfur oxides (SOx) and nitrogen oxides (NOx) toxic gases and to prevent air pollution.
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U2 - 10.1016/j.ijbiomac.2019.11.234
DO - 10.1016/j.ijbiomac.2019.11.234
M3 - Article
C2 - 31794827
AN - SCOPUS:85076604538
SN - 0141-8130
VL - 152
SP - 930
EP - 938
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -