Crowning proteins: Modulating the protein surface properties using crown ethers

Cheng Chung Lee, Manuel Maestre-Reyna, Kai Cheng Hsu, Hao Ching Wang, Chia I. Liu, Wen Yih Jeng, Li Ling Lin, Richard Wood, Chia Cheng Chou, Jinn Moon Yang, Andrew H.J. Wang

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Crown ethers are small, cyclic polyethers that have found wide-spread use in phase-transfer catalysis and, to a certain degree, in protein chemistry. Crown ethers readily bind metallic and organic cations, including positively charged amino acid side chains. We elucidated the crystal structures of several protein-crown ether co-crystals grown in the presence of 18-crown-6. We then employed biophysical methods and molecular dynamics simulations to compare these complexes with the corresponding apoproteins and with similar complexes with ring-shaped low-molecular-weight polyethylene glycols. Our studies show that crown ethers can modify protein surface behavior dramatically by stabilizing either intra- or intermolecular interactions. Consequently, we propose that crown ethers can be used to modulate a wide variety of protein surface behaviors, such as oligomerization, domain-domain interactions, stabilization in organic solvents, and crystallization.

Original languageEnglish
Pages (from-to)13054-13058
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number48
DOIs
Publication statusPublished - 2014 Nov 24

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

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