Crowning proteins: Modulating the protein surface properties using crown ethers

Cheng Chung Lee, Manuel Maestre-Reyna, Kai Cheng Hsu, Hao Ching Wang, Chia I. Liu, Wen Yih Jeng, Li Ling Lin, Richard Wood, Chia Cheng Chou, Jinn Moon Yang, Andrew H.J. Wang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Crown ethers are small, cyclic polyethers that have found wide-spread use in phase-transfer catalysis and, to a certain degree, in protein chemistry. Crown ethers readily bind metallic and organic cations, including positively charged amino acid side chains. We elucidated the crystal structures of several protein-crown ether co-crystals grown in the presence of 18-crown-6. We then employed biophysical methods and molecular dynamics simulations to compare these complexes with the corresponding apoproteins and with similar complexes with ring-shaped low-molecular-weight polyethylene glycols. Our studies show that crown ethers can modify protein surface behavior dramatically by stabilizing either intra- or intermolecular interactions. Consequently, we propose that crown ethers can be used to modulate a wide variety of protein surface behaviors, such as oligomerization, domain-domain interactions, stabilization in organic solvents, and crystallization.

Original languageEnglish
Pages (from-to)13054-13058
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number48
DOIs
Publication statusPublished - 2014 Nov 24

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Crown Ethers
Crown ethers
Surface properties
Proteins
Membrane Proteins
Oligomerization
Apoproteins
Polyethers
Crystallization
Organic solvents
Catalysis
Polyethylene glycols
Molecular dynamics
Cations
Amino acids
Stabilization
Crystal structure
Positive ions
Molecular weight
Amino Acids

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Cite this

Lee, C. C., Maestre-Reyna, M., Hsu, K. C., Wang, H. C., Liu, C. I., Jeng, W. Y., ... Wang, A. H. J. (2014). Crowning proteins: Modulating the protein surface properties using crown ethers. Angewandte Chemie - International Edition, 53(48), 13054-13058. https://doi.org/10.1002/anie.201405664
Lee, Cheng Chung ; Maestre-Reyna, Manuel ; Hsu, Kai Cheng ; Wang, Hao Ching ; Liu, Chia I. ; Jeng, Wen Yih ; Lin, Li Ling ; Wood, Richard ; Chou, Chia Cheng ; Yang, Jinn Moon ; Wang, Andrew H.J. / Crowning proteins : Modulating the protein surface properties using crown ethers. In: Angewandte Chemie - International Edition. 2014 ; Vol. 53, No. 48. pp. 13054-13058.
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Lee, CC, Maestre-Reyna, M, Hsu, KC, Wang, HC, Liu, CI, Jeng, WY, Lin, LL, Wood, R, Chou, CC, Yang, JM & Wang, AHJ 2014, 'Crowning proteins: Modulating the protein surface properties using crown ethers', Angewandte Chemie - International Edition, vol. 53, no. 48, pp. 13054-13058. https://doi.org/10.1002/anie.201405664

Crowning proteins : Modulating the protein surface properties using crown ethers. / Lee, Cheng Chung; Maestre-Reyna, Manuel; Hsu, Kai Cheng; Wang, Hao Ching; Liu, Chia I.; Jeng, Wen Yih; Lin, Li Ling; Wood, Richard; Chou, Chia Cheng; Yang, Jinn Moon; Wang, Andrew H.J.

In: Angewandte Chemie - International Edition, Vol. 53, No. 48, 24.11.2014, p. 13054-13058.

Research output: Contribution to journalArticle

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AU - Lee, Cheng Chung

AU - Maestre-Reyna, Manuel

AU - Hsu, Kai Cheng

AU - Wang, Hao Ching

AU - Liu, Chia I.

AU - Jeng, Wen Yih

AU - Lin, Li Ling

AU - Wood, Richard

AU - Chou, Chia Cheng

AU - Yang, Jinn Moon

AU - Wang, Andrew H.J.

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