TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic analysis of thioesterase I from Escherichia coli
AU - Lo, Yu Chih
AU - Lee, Ya Lin
AU - Shaw, Jei Fu
AU - Liaw, Yen Chywan
PY - 2000/7/3
Y1 - 2000/7/3
N2 - The Escherichia coli thioesterase I specifically catalyzes the deacylation of fatty acyl-CoA thioesters, especially those with long acyl groups (C12-C18). Single crystals of thioesterase I (E.C. 3.1.2.2) from E. coli have been obtained using methoxypolyethylene glycol 5000 (PEG-MME 5K) as a precipitant at room temperature in 21 d. The crystals belong to the tetragonal space group P41212 or its enantiomorph P43212, with unit-cell parameters a = b = 50.85 (7), c = 171.5 (1) Å. The crystals diffract to beyond 2.4 Å resolution. There is one molecule of molecular weight 20.5 kDa in the asymmetric unit, with a solvent content of 55%.
AB - The Escherichia coli thioesterase I specifically catalyzes the deacylation of fatty acyl-CoA thioesters, especially those with long acyl groups (C12-C18). Single crystals of thioesterase I (E.C. 3.1.2.2) from E. coli have been obtained using methoxypolyethylene glycol 5000 (PEG-MME 5K) as a precipitant at room temperature in 21 d. The crystals belong to the tetragonal space group P41212 or its enantiomorph P43212, with unit-cell parameters a = b = 50.85 (7), c = 171.5 (1) Å. The crystals diffract to beyond 2.4 Å resolution. There is one molecule of molecular weight 20.5 kDa in the asymmetric unit, with a solvent content of 55%.
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U2 - 10.1107/S0907444900004339
DO - 10.1107/S0907444900004339
M3 - Article
C2 - 10818355
AN - SCOPUS:0034040846
VL - 56
SP - 756
EP - 757
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
SN - 0907-4449
IS - 6
ER -