d-2-amino-5-phosphonovaleate blocks induction of long-term depression of the NMDA receptor-mediated synaptic component in rat hippocampus

Po-Wu Gean, Jju Home Lin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The long-term depression (LTD) of pharmacologically isolated N-methyl-d-aspartate (NMDA) receptor-mediated excitatory postsynaptic potential (EPSPNMDA) was studied in an vitro slice preparation of rat hippocampus. Intracellular recordings were made from CA1 pyramidal cells in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and picrotoxin (50 μM) which block non-NMDA and GABAA receptors, respectively. Low frequency (1 Hz) synaptic stimulation caused a transient decrease in the amplitude of EPSPNMDA that usually restored to its control level within 15 min after the stimulation. However, pairing of low frequency synaptic stimulation with postsynaptic depolarization induced an LTD of EPSPNMDA. The EPSPNMDA LTD could be blocked by d-2-amino-5-phosphonovaleate (d-APV, 20 μM) suggesting that the induction of LTD requires an increase in postsynaptic Ca2+, at least in part, due to synaptic activation of NMDA receptors during concomitant postsynaptic depolarization.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalNeuroscience Letters
Volume158
Issue number2
DOIs
Publication statusPublished - 1993 Aug 20

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6-Cyano-7-nitroquinoxaline-2,3-dione
Hippocampus
2-Amino-5-phosphonovalerate
Picrotoxin
Pyramidal Cells
Excitatory Postsynaptic Potentials
GABA-A Receptors
aspartic acid receptor
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

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title = "d-2-amino-5-phosphonovaleate blocks induction of long-term depression of the NMDA receptor-mediated synaptic component in rat hippocampus",
abstract = "The long-term depression (LTD) of pharmacologically isolated N-methyl-d-aspartate (NMDA) receptor-mediated excitatory postsynaptic potential (EPSPNMDA) was studied in an vitro slice preparation of rat hippocampus. Intracellular recordings were made from CA1 pyramidal cells in the presence of 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM) and picrotoxin (50 μM) which block non-NMDA and GABAA receptors, respectively. Low frequency (1 Hz) synaptic stimulation caused a transient decrease in the amplitude of EPSPNMDA that usually restored to its control level within 15 min after the stimulation. However, pairing of low frequency synaptic stimulation with postsynaptic depolarization induced an LTD of EPSPNMDA. The EPSPNMDA LTD could be blocked by d-2-amino-5-phosphonovaleate (d-APV, 20 μM) suggesting that the induction of LTD requires an increase in postsynaptic Ca2+, at least in part, due to synaptic activation of NMDA receptors during concomitant postsynaptic depolarization.",
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d-2-amino-5-phosphonovaleate blocks induction of long-term depression of the NMDA receptor-mediated synaptic component in rat hippocampus. / Gean, Po-Wu; Lin, Jju Home.

In: Neuroscience Letters, Vol. 158, No. 2, 20.08.1993, p. 170-172.

Research output: Contribution to journalArticle

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