d-myo-Inositol 1,4,5-trisphosphate analogues as useful tools in biochemical studies of intracellular calcium mobilization

Da Ming Gou, Woan Ru Shieh, Pei-Jung Lu, Ching Shih Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Two types of structural variants of d-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] were prepared by a chemo-enzymatic route. These 6-O-substituted analogues retained the biological activity of Ins(1,4,5)P3, and were able to elicit Ca2+ release from porcine brain microsomes. Moreover, these derivatives allowed the preparation of Ins(1,4,5)P3-based immunogens and affinity matrix which were successfully applied to the preparation and purification of antibodies against Ins(1,4,5)P3. These antibodies displayed discriminative affinity towards Ins(1,4,5)P3, and provide a useful tool to study intracellular Ca2+ mobilization.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalBioorganic and Medicinal Chemistry
Volume2
Issue number1
DOIs
Publication statusPublished - 1994 Jan 1

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Inositol 1,4,5-Trisphosphate
Calcium
Antibodies
Microsomes
Bioactivity
Purification
Brain
Swine
Derivatives

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

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abstract = "Two types of structural variants of d-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] were prepared by a chemo-enzymatic route. These 6-O-substituted analogues retained the biological activity of Ins(1,4,5)P3, and were able to elicit Ca2+ release from porcine brain microsomes. Moreover, these derivatives allowed the preparation of Ins(1,4,5)P3-based immunogens and affinity matrix which were successfully applied to the preparation and purification of antibodies against Ins(1,4,5)P3. These antibodies displayed discriminative affinity towards Ins(1,4,5)P3, and provide a useful tool to study intracellular Ca2+ mobilization.",
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d-myo-Inositol 1,4,5-trisphosphate analogues as useful tools in biochemical studies of intracellular calcium mobilization. / Gou, Da Ming; Shieh, Woan Ru; Lu, Pei-Jung; Chen, Ching Shih.

In: Bioorganic and Medicinal Chemistry, Vol. 2, No. 1, 01.01.1994, p. 7-13.

Research output: Contribution to journalArticle

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