d-myo-Inositol 1,4,5-trisphosphate analogues as useful tools in biochemical studies of intracellular calcium mobilization

Da Ming Gou; Woan Ru Shieh; Pei Jung Lu; Ching Shih Chen

doi:10.1016/S0968-0896(00)82196-3

d-myo-Inositol 1,4,5-trisphosphate analogues as useful tools in biochemical studies of intracellular calcium mobilization

Da Ming Gou, Woan Ru Shieh, Pei Jung Lu, Ching Shih Chen

Institute of Clinical Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Two types of structural variants of d-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P₃] were prepared by a chemo-enzymatic route. These 6-O-substituted analogues retained the biological activity of Ins(1,4,5)P₃, and were able to elicit Ca²⁺ release from porcine brain microsomes. Moreover, these derivatives allowed the preparation of Ins(1,4,5)P₃-based immunogens and affinity matrix which were successfully applied to the preparation and purification of antibodies against Ins(1,4,5)P₃. These antibodies displayed discriminative affinity towards Ins(1,4,5)P₃, and provide a useful tool to study intracellular Ca²⁺ mobilization.

Original language	English
Pages (from-to)	7-13
Number of pages	7
Journal	Bioorganic and Medicinal Chemistry
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/S0968-0896(00)82196-3
Publication status	Published - 1994 Jan

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/S0968-0896(00)82196-3

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title = "d-myo-Inositol 1,4,5-trisphosphate analogues as useful tools in biochemical studies of intracellular calcium mobilization",

abstract = "Two types of structural variants of d-myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] were prepared by a chemo-enzymatic route. These 6-O-substituted analogues retained the biological activity of Ins(1,4,5)P3, and were able to elicit Ca2+ release from porcine brain microsomes. Moreover, these derivatives allowed the preparation of Ins(1,4,5)P3-based immunogens and affinity matrix which were successfully applied to the preparation and purification of antibodies against Ins(1,4,5)P3. These antibodies displayed discriminative affinity towards Ins(1,4,5)P3, and provide a useful tool to study intracellular Ca2+ mobilization.",

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AU - Shieh, Woan Ru

AU - Lu, Pei Jung

AU - Chen, Ching Shih

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d-myo-Inositol 1,4,5-trisphosphate analogues as useful tools in biochemical studies of intracellular calcium mobilization

Abstract

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