TY - JOUR
T1 - Dark fermentative hydrogen production from enzymatic hydrolysate of xylan and pretreated rice straw by Clostridium butyricum CGS5
AU - Lo, Yung Chung
AU - Lu, Wei Chung
AU - Chen, Chun Yen
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial supports from Taiwan’s National Science Council (Grant Nos. NSC-96-2218-E-006-295 , NSC-96-2628-E-006-004-MY3 , 97-2218-E-006-005 , and 98-2218-E-006-002 ) as well as from National Cheng Kung University (Landmark Program, Project No. A029).
PY - 2010/8
Y1 - 2010/8
N2 - Xylan and rice straw were used to produce H2 via a two-stage approach combining feedstock pretreatment/hydrolysis and dark H2 fermentation. Acinetobacter junii F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. The hydrolysates were converted to H2 by Clostridium butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan and NaOH-pretreated rice straw shows νmax values of 8.6 and 3.6 g/L/h, and Km values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5 and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw, respectively, while the hydrogen yield was 0.70 and 0.76 mol H2/mol xylose, respectively. Simultaneous saccharification and BioH2 fermentation from xylan was also conducted but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage process.
AB - Xylan and rice straw were used to produce H2 via a two-stage approach combining feedstock pretreatment/hydrolysis and dark H2 fermentation. Acinetobacter junii F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. The hydrolysates were converted to H2 by Clostridium butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan and NaOH-pretreated rice straw shows νmax values of 8.6 and 3.6 g/L/h, and Km values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5 and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw, respectively, while the hydrogen yield was 0.70 and 0.76 mol H2/mol xylose, respectively. Simultaneous saccharification and BioH2 fermentation from xylan was also conducted but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage process.
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U2 - 10.1016/j.biortech.2010.02.085
DO - 10.1016/j.biortech.2010.02.085
M3 - Article
C2 - 20385486
AN - SCOPUS:77951090497
SN - 0960-8524
VL - 101
SP - 5885
EP - 5891
JO - Bioresource technology
JF - Bioresource technology
IS - 15
ER -