Dark fermentative hydrogen production from enzymatic hydrolysate of xylan and pretreated rice straw by Clostridium butyricum CGS5

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Abstract

Xylan and rice straw were used to produce H2 via a two-stage approach combining feedstock pretreatment/hydrolysis and dark H2 fermentation. Acinetobacter junii F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. The hydrolysates were converted to H2 by Clostridium butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan and NaOH-pretreated rice straw shows νmax values of 8.6 and 3.6 g/L/h, and Km values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5 and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw, respectively, while the hydrogen yield was 0.70 and 0.76 mol H2/mol xylose, respectively. Simultaneous saccharification and BioH2 fermentation from xylan was also conducted but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage process.

Original languageEnglish
Pages (from-to)5885-5891
Number of pages7
JournalBioresource technology
Volume101
Issue number15
DOIs
Publication statusPublished - 2010 Aug 1

Fingerprint

Xylans
Clostridium
Straw
Hydrogen production
straw
rice
hydrogen
fermentation
Fermentation
Saccharification
Enzyme kinetics
Xylose
hydrolysis
Feedstocks
enzyme
Hydrogen
Hydrolysis
kinetics
Enzymes
rate

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

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title = "Dark fermentative hydrogen production from enzymatic hydrolysate of xylan and pretreated rice straw by Clostridium butyricum CGS5",
abstract = "Xylan and rice straw were used to produce H2 via a two-stage approach combining feedstock pretreatment/hydrolysis and dark H2 fermentation. Acinetobacter junii F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. The hydrolysates were converted to H2 by Clostridium butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan and NaOH-pretreated rice straw shows νmax values of 8.6 and 3.6 g/L/h, and Km values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5 and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw, respectively, while the hydrogen yield was 0.70 and 0.76 mol H2/mol xylose, respectively. Simultaneous saccharification and BioH2 fermentation from xylan was also conducted but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage process.",
author = "Lo, {Yung Chung} and Lu, {Wei Chung} and Chen, {Chun Yen} and Chang, {Jo Shu}",
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AU - Lo, Yung Chung

AU - Lu, Wei Chung

AU - Chen, Chun Yen

AU - Chang, Jo Shu

PY - 2010/8/1

Y1 - 2010/8/1

N2 - Xylan and rice straw were used to produce H2 via a two-stage approach combining feedstock pretreatment/hydrolysis and dark H2 fermentation. Acinetobacter junii F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. The hydrolysates were converted to H2 by Clostridium butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan and NaOH-pretreated rice straw shows νmax values of 8.6 and 3.6 g/L/h, and Km values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5 and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw, respectively, while the hydrogen yield was 0.70 and 0.76 mol H2/mol xylose, respectively. Simultaneous saccharification and BioH2 fermentation from xylan was also conducted but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage process.

AB - Xylan and rice straw were used to produce H2 via a two-stage approach combining feedstock pretreatment/hydrolysis and dark H2 fermentation. Acinetobacter junii F6-02 was used to produce cellulolytic enzymes (mainly xylanase) to hydrolyze xylan and pretreated rice straw. The hydrolysates were converted to H2 by Clostridium butyricum CGS5 via dark fermentation. Investigation of kinetics of xylanase on xylan and NaOH-pretreated rice straw shows νmax values of 8.6 and 3.6 g/L/h, and Km values of 10.6 and 26.9 g/L, respectively. A maximum hydrogen production rate of 62.5 and 26.8 ml/h/L was obtained from hydrolysate of xylan and pretreated rice straw, respectively, while the hydrogen yield was 0.70 and 0.76 mol H2/mol xylose, respectively. Simultaneous saccharification and BioH2 fermentation from xylan was also conducted but giving a lower hydrogen production rate (35.3 ml/h/L) than that of the two-stage process.

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