Dark fermentative hydrogen production with crude glycerol from biodiesel industry using indigenous hydrogen-producing bacteria

Yung Chung Lo, Xue Jiao Chen, Chi Yu Huang, Ying Jin Yuan, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

Glycerol is an inevitable by-product from biodiesel synthesis process and could be a promising feedstock for fermentative hydrogen production. In this study, the feasibility of using crude glycerol from biodiesel industry for biohydrogen production was evaluated using seven isolated hydrogen-producing bacterial strains (Clostridium butyricum, Clostridium pasteurianum, and Klebsiella sp.). Among the strains examined, C. pasteurianum CH4 exhibited the best biohydrogen-producing performance under the optimal conditions of: temperature, 35 C; initial pH, 7.0; agitation rate, 200 rpm; glycerol concentration, 10 g/l. When using pure glycerol as carbon source for continuous hydrogen fermentation, the average H2 production rate and H 2 yield were 103.1 ± 8.1 ml/h/l and 0.50 ± 0.02 mol H2/mol glycerol, respectively. In contrast, when using crude glycerol as the carbon source, the H2 production rate and H2 yield was improved to 166.0 ± 8.7 ml/h/l and 0.77 ± 0.05 mol H 2/mol glycerol, respectively. This work demonstrated the high potential of using biodiesel by-product, glycerol, for cost-effective biohydrogen production.

Original languageEnglish
Pages (from-to)15815-15822
Number of pages8
JournalInternational Journal of Hydrogen Energy
Volume38
Issue number35
DOIs
Publication statusPublished - 2013 Nov 22

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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