DAZL protein expression in mouse preimplantation embryo

Hsien An Pan, Rui Wen Liao, Chia Ling Chung, Yen Ni Teng, YungMing Lin, Pao-Lin Kuo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: To evaluate the expression pattern of Dazl (deleted in azoospermia-like) protein in the mouse preimplantation embryo. Design: Experimental study. Setting: Medical research laboratory in a university hospital. Animal(s): Twenty female 28- to 35-day-old FVB mice. Intervention(s): Embryo collection at 1.5, 2.5, and 3.5 days postcoitus (plug date, 0.5 d postcoitus) to examine the Dazl protein expression from the two-cell embryo to the blastocyst. Main Outcome Measure(s): Dazl protein expression was analyzed by immunofluorescent staining. Result(s): There is abundant expression of Dazl protein in the cytoplasm of the blastomere. Strong fluorescent signals of Dazl protein expression were found in preimplantation embryo cytoplasm, including two-cell, eight-cell, morula, and blastocyst. Conclusion(s): By using an antibody raised against mouse Daz-like protein (Dazl), we showed that Dazl protein is present in all cleaving stages of the preimplantation embryo. This is the first report on the protein expression of a Dazl gene during embryogenesis in mice. However, further study is needed to evaluate the molecular functional role of Dazl.

Original languageEnglish
Pages (from-to)1324-1327
Number of pages4
JournalFertility and Sterility
Volume89
Issue number5 SUPPL.
DOIs
Publication statusPublished - 2008 May 1

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Blastocyst
Azoospermia
Proteins
Cytoplasm
Embryonic Structures
Morula
Blastomeres
Embryonic Development
Biomedical Research
Research Design
Outcome Assessment (Health Care)
human DAZ1 protein
Staining and Labeling
Antibodies
Genes

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cite this

Pan, H. A., Liao, R. W., Chung, C. L., Teng, Y. N., Lin, Y., & Kuo, P-L. (2008). DAZL protein expression in mouse preimplantation embryo. Fertility and Sterility, 89(5 SUPPL.), 1324-1327. https://doi.org/10.1016/j.fertnstert.2007.03.041
Pan, Hsien An ; Liao, Rui Wen ; Chung, Chia Ling ; Teng, Yen Ni ; Lin, YungMing ; Kuo, Pao-Lin. / DAZL protein expression in mouse preimplantation embryo. In: Fertility and Sterility. 2008 ; Vol. 89, No. 5 SUPPL. pp. 1324-1327.
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Pan, HA, Liao, RW, Chung, CL, Teng, YN, Lin, Y & Kuo, P-L 2008, 'DAZL protein expression in mouse preimplantation embryo', Fertility and Sterility, vol. 89, no. 5 SUPPL., pp. 1324-1327. https://doi.org/10.1016/j.fertnstert.2007.03.041

DAZL protein expression in mouse preimplantation embryo. / Pan, Hsien An; Liao, Rui Wen; Chung, Chia Ling; Teng, Yen Ni; Lin, YungMing; Kuo, Pao-Lin.

In: Fertility and Sterility, Vol. 89, No. 5 SUPPL., 01.05.2008, p. 1324-1327.

Research output: Contribution to journalArticle

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AU - Pan, Hsien An

AU - Liao, Rui Wen

AU - Chung, Chia Ling

AU - Teng, Yen Ni

AU - Lin, YungMing

AU - Kuo, Pao-Lin

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