Decolorization kinetics of a recombinant Escherichia coli strain harboring azo-dye-decolorizing determinants from Rhodococcus sp.

Jo-Shu Chang, Chia Yu Lin

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A 6.3 kb DNA fragment containing genes responsible for azo-dye decolorization was cloned and expressed in Escherichia coli. The resulting recombinant strain E. coli CY1 decolorized 200 mg azo dye (C.I. Reactive Red 22) 1 -1 at 28 °C at 8.2 mg g cell -1 h -1 while the host (E. coli DH5α) had no color-removal activity. Addition of 0.5 mM isopropyl-β-D-thiogalacto-pyranoside (IPTG) increased the decolorization rate 3.4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye at 600 mg 1 -1. The decolorization rate of E. coli CY1 was optimal at 40°C and pH 11. Aeration (increased dissolved O 2 level) strongly inhibited the decolorization, but decolorization occurred effectively under static incubation conditions (no agitation was employed). The CY1 strain also exhibited excellent stability during repeated-batch operations.

Original languageEnglish
Pages (from-to)631-636
Number of pages6
JournalBiotechnology Letters
Volume23
Issue number8
DOIs
Publication statusPublished - 2001 May 10

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Azo Compounds
Rhodococcus
Azo dyes
Escherichia coli
Kinetics
Coloring Agents
Dyes
DNA
Color
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

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title = "Decolorization kinetics of a recombinant Escherichia coli strain harboring azo-dye-decolorizing determinants from Rhodococcus sp.",
abstract = "A 6.3 kb DNA fragment containing genes responsible for azo-dye decolorization was cloned and expressed in Escherichia coli. The resulting recombinant strain E. coli CY1 decolorized 200 mg azo dye (C.I. Reactive Red 22) 1 -1 at 28 °C at 8.2 mg g cell -1 h -1 while the host (E. coli DH5α) had no color-removal activity. Addition of 0.5 mM isopropyl-β-D-thiogalacto-pyranoside (IPTG) increased the decolorization rate 3.4-fold. The dependence of the decolorization rate on initial dye concentration essentially followed Monod-type kinetics and the maximal rate occurred with the dye at 600 mg 1 -1. The decolorization rate of E. coli CY1 was optimal at 40°C and pH 11. Aeration (increased dissolved O 2 level) strongly inhibited the decolorization, but decolorization occurred effectively under static incubation conditions (no agitation was employed). The CY1 strain also exhibited excellent stability during repeated-batch operations.",
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Decolorization kinetics of a recombinant Escherichia coli strain harboring azo-dye-decolorizing determinants from Rhodococcus sp. / Chang, Jo-Shu; Lin, Chia Yu.

In: Biotechnology Letters, Vol. 23, No. 8, 10.05.2001, p. 631-636.

Research output: Contribution to journalArticle

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