Decrease of fibrinolytic activity in human endothelial cells by arsenite

Shinn Jong Jiang, Tsun Mei Lin, Hua-Lin Wu, Huai Song Han, Guey Yueh Shi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Blackfoot disease (BFD) is an endemic peripheral vascular occlusive disease that occurred in the southwest coast of Taiwan. It is believed that arsenic in the drinking water from artesian wells plays an important role in the development of the disease. We have previously shown that BFD patients had significant lower tissue-type plasminogen activator (t-PA) antigen level and higher plasminogen activator inhibitor, Type 1 (PAI-1) antigen level than normal controls. The purpose of this study was to investigate the effects of arsenite on the fibrinolytic and anticoagulant activities of cultured macrovascular and microvascular endothelial cells. Incubation of human microvascular endothelial cells (HMEC-1), but not human umbilical vein endothelial cells (HUVECs), with arsenite caused a decrease of t-PA mRNA level, a rise of both PAI-1 mRNA level and PAI activity. Arsenite could also inhibit the thrombomodulin (TM) mRNA expression and reduce the TM antigen level in HMEC-1. In conclusion, arsenite had a greater effect on HMEC-1 as compared to HUVECs in lowering the fibrinolytic activity and may be responsible for the reduced capacity of fibrinolysis associated with BFD.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalThrombosis Research
Volume105
Issue number1
DOIs
Publication statusPublished - 2002 Jan 1

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Human Activities
Endothelial Cells
Thrombomodulin
Plasminogen Activator Inhibitor 1
Human Umbilical Vein Endothelial Cells
Antigens
Messenger RNA
Water Wells
Endemic Diseases
Peripheral Vascular Diseases
Plasminogen Activators
Arsenic
Fibrinolysis
Tissue Plasminogen Activator
Taiwan
Drinking Water
Anticoagulants
arsenite

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Jiang, Shinn Jong ; Lin, Tsun Mei ; Wu, Hua-Lin ; Han, Huai Song ; Shi, Guey Yueh. / Decrease of fibrinolytic activity in human endothelial cells by arsenite. In: Thrombosis Research. 2002 ; Vol. 105, No. 1. pp. 55-62.
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Decrease of fibrinolytic activity in human endothelial cells by arsenite. / Jiang, Shinn Jong; Lin, Tsun Mei; Wu, Hua-Lin; Han, Huai Song; Shi, Guey Yueh.

In: Thrombosis Research, Vol. 105, No. 1, 01.01.2002, p. 55-62.

Research output: Contribution to journalArticle

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