TY - JOUR
T1 - Decreased mRNA transcripts of M-phase promoting factor and its regulators in the testes of infertile men
AU - Lin, Yung Ming
AU - Teng, Yen Ni
AU - Chung, Chia Ling
AU - Tsai, Wan Ching
AU - Lin, Ying Hung
AU - Lin, Johnny Shinn Nan
AU - Kuo, Pao Lin
N1 - Funding Information:
This study is supported by grants from the National Science Council of Taiwan (NSC 91–2314-B-006–149, NSC 91–3112-B-006–008, NSC 92–3112-B-006–002, NSC 93–3112-B-006–004, NSC 93– 2314-B-006–69 and NSC 93–2314-B-006–078).
PY - 2006/1
Y1 - 2006/1
N2 - Background: M-phase promoting factor (MPF), which is comprised of Cyclin B and a catalytic subunit, Cdc2, is a key enzyme required for cells to enter M phase in both mitosis and meiosis. MPF activity is controlled by the stimulatory dephosphorylation of the Cdc25 family and the inhibitory phosphorylation of Wee1. We determined the levels of mRNA transcripts of MPF and its regulators in the testes of infertile men, and evaluated the relationship between the transcript levels and patients' testicular phenotypes and sperm retrieval results. Methods and results: The mRNA transcript levels of CDC2, CCNB1, CCNB2, CDC25A, CDC25B, CDC25C and WEE1 in the testes of 37 azoospermic patients were examined by quantitative real-time polymerase chain reaction. Significant decreases in CDC2, CCNB1, CCNB2, CDC25A, CDC25C and WEE1 mRNA transcript levels were detected in patients with spermatogenic failure. CDC2 mRNA transcript levels correlated significantly with those of CCNB1 and CCNB2 mRNA. Significantly higher CDC2, CCNB1, CCNB2, CDC25C and WEE1 mRNA transcript levels were detected in 18 patients with successful sperm retrieval than in 11 patients with failed sperm retrieval. Conclusions: We suggest that the decreased mRNA transcripts of MPF and its regulators play important roles in human spermatogenesis.
AB - Background: M-phase promoting factor (MPF), which is comprised of Cyclin B and a catalytic subunit, Cdc2, is a key enzyme required for cells to enter M phase in both mitosis and meiosis. MPF activity is controlled by the stimulatory dephosphorylation of the Cdc25 family and the inhibitory phosphorylation of Wee1. We determined the levels of mRNA transcripts of MPF and its regulators in the testes of infertile men, and evaluated the relationship between the transcript levels and patients' testicular phenotypes and sperm retrieval results. Methods and results: The mRNA transcript levels of CDC2, CCNB1, CCNB2, CDC25A, CDC25B, CDC25C and WEE1 in the testes of 37 azoospermic patients were examined by quantitative real-time polymerase chain reaction. Significant decreases in CDC2, CCNB1, CCNB2, CDC25A, CDC25C and WEE1 mRNA transcript levels were detected in patients with spermatogenic failure. CDC2 mRNA transcript levels correlated significantly with those of CCNB1 and CCNB2 mRNA. Significantly higher CDC2, CCNB1, CCNB2, CDC25C and WEE1 mRNA transcript levels were detected in 18 patients with successful sperm retrieval than in 11 patients with failed sperm retrieval. Conclusions: We suggest that the decreased mRNA transcripts of MPF and its regulators play important roles in human spermatogenesis.
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U2 - 10.1093/humrep/dei285
DO - 10.1093/humrep/dei285
M3 - Article
C2 - 16155078
AN - SCOPUS:29144447446
VL - 21
SP - 138
EP - 144
JO - Human Reproduction
JF - Human Reproduction
SN - 0268-1161
IS - 1
ER -