Decreased mRNA transcripts of M-phase promoting factor and its regulators in the testes of infertile men

Yung Ming Lin, Yen Ni Teng, Chia Ling Chung, Wan Ching Tsai, Ying Hung Lin, Johnny Shinn Nan Lin, Pao Lin Kuo

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background: M-phase promoting factor (MPF), which is comprised of Cyclin B and a catalytic subunit, Cdc2, is a key enzyme required for cells to enter M phase in both mitosis and meiosis. MPF activity is controlled by the stimulatory dephosphorylation of the Cdc25 family and the inhibitory phosphorylation of Wee1. We determined the levels of mRNA transcripts of MPF and its regulators in the testes of infertile men, and evaluated the relationship between the transcript levels and patients' testicular phenotypes and sperm retrieval results. Methods and results: The mRNA transcript levels of CDC2, CCNB1, CCNB2, CDC25A, CDC25B, CDC25C and WEE1 in the testes of 37 azoospermic patients were examined by quantitative real-time polymerase chain reaction. Significant decreases in CDC2, CCNB1, CCNB2, CDC25A, CDC25C and WEE1 mRNA transcript levels were detected in patients with spermatogenic failure. CDC2 mRNA transcript levels correlated significantly with those of CCNB1 and CCNB2 mRNA. Significantly higher CDC2, CCNB1, CCNB2, CDC25C and WEE1 mRNA transcript levels were detected in 18 patients with successful sperm retrieval than in 11 patients with failed sperm retrieval. Conclusions: We suggest that the decreased mRNA transcripts of MPF and its regulators play important roles in human spermatogenesis.

Original languageEnglish
Pages (from-to)138-144
Number of pages7
JournalHuman Reproduction
Volume21
Issue number1
DOIs
Publication statusPublished - 2006 Jan

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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