Deficiency of electroneutral K+-Cl- cotransporter 3 causes a disruption in impulse propagation along peripheral nerves

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Abstract

Nerve conduction requires the fine tuning of ionic currents through delicate interactions between axons and Schwann cells. The K+-Cl - cotransporter (KCC) family includes four isoforms (KCC1-4) that play an important role in the maintenance of cellular osmotic homeostasis via the coupled electroneutral movement of K+ and Cl- with concurrent water flux. Mutation in SLC12A6 gene encoding KCC3 results in an autosomal recessive disease, known as agenesis of the corpus callosum associated with peripheral neuropathy. Nevertheless, the role of KCC3 in nerve function remains a puzzle. In this study, the microscopic examination of KCC isoforms expressed in peripheral nerves showed high expression of KCC2-4 in nodal segments of the axons and in the perinucleus and microvilli of Schwann cells. The KCC inhibitor [[(dihydroindenyl)oxy]alkanoic acid] but not the Na +-K+-2Cl--cotransport inhibitor (bumetanide) dose-dependently suppressed the amplitude and area of compound muscle action potential, indicating the involvement of KCC activity in peripheral nerve conduction. Furthermore, the amplitude and area under the curve were smaller, and the nerve conduction velocity was slower in nerves from KCC3-/- mice than in nerves from wild-type mice, while the expression pattern of KCC2 and KCC4 was similar in KCC3 kockout and wild-type strains. KCC3-/- mice also manifested a prominent motor deficit in the beam-walking test. This is the first study to demonstrate that the K+-Cl- cotransporter activity of KCC3 contributes to the propagation of action potentials along peripheral nerves.

Original languageEnglish
Pages (from-to)1544-1552
Number of pages9
JournalGLIA
Volume58
Issue number13
DOIs
Publication statusPublished - 2010 Oct 1

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Peripheral Nerves
Neural Conduction
Schwann Cells
Action Potentials
Axons
Protein Isoforms
Bumetanide
Microvilli
Walking
Area Under Curve
Homeostasis
Maintenance
potassium-chloride symporters
Muscles
Mutation
Water
Genes

All Science Journal Classification (ASJC) codes

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

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title = "Deficiency of electroneutral K+-Cl- cotransporter 3 causes a disruption in impulse propagation along peripheral nerves",
abstract = "Nerve conduction requires the fine tuning of ionic currents through delicate interactions between axons and Schwann cells. The K+-Cl - cotransporter (KCC) family includes four isoforms (KCC1-4) that play an important role in the maintenance of cellular osmotic homeostasis via the coupled electroneutral movement of K+ and Cl- with concurrent water flux. Mutation in SLC12A6 gene encoding KCC3 results in an autosomal recessive disease, known as agenesis of the corpus callosum associated with peripheral neuropathy. Nevertheless, the role of KCC3 in nerve function remains a puzzle. In this study, the microscopic examination of KCC isoforms expressed in peripheral nerves showed high expression of KCC2-4 in nodal segments of the axons and in the perinucleus and microvilli of Schwann cells. The KCC inhibitor [[(dihydroindenyl)oxy]alkanoic acid] but not the Na +-K+-2Cl--cotransport inhibitor (bumetanide) dose-dependently suppressed the amplitude and area of compound muscle action potential, indicating the involvement of KCC activity in peripheral nerve conduction. Furthermore, the amplitude and area under the curve were smaller, and the nerve conduction velocity was slower in nerves from KCC3-/- mice than in nerves from wild-type mice, while the expression pattern of KCC2 and KCC4 was similar in KCC3 kockout and wild-type strains. KCC3-/- mice also manifested a prominent motor deficit in the beam-walking test. This is the first study to demonstrate that the K+-Cl- cotransporter activity of KCC3 contributes to the propagation of action potentials along peripheral nerves.",
author = "Yuan-Ting Sun and Thy-Sheng Lin and Shun-Fen Tzeng and Eric Delpire and Meng-Ru Shen",
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T1 - Deficiency of electroneutral K+-Cl- cotransporter 3 causes a disruption in impulse propagation along peripheral nerves

AU - Sun, Yuan-Ting

AU - Lin, Thy-Sheng

AU - Tzeng, Shun-Fen

AU - Delpire, Eric

AU - Shen, Meng-Ru

PY - 2010/10/1

Y1 - 2010/10/1

N2 - Nerve conduction requires the fine tuning of ionic currents through delicate interactions between axons and Schwann cells. The K+-Cl - cotransporter (KCC) family includes four isoforms (KCC1-4) that play an important role in the maintenance of cellular osmotic homeostasis via the coupled electroneutral movement of K+ and Cl- with concurrent water flux. Mutation in SLC12A6 gene encoding KCC3 results in an autosomal recessive disease, known as agenesis of the corpus callosum associated with peripheral neuropathy. Nevertheless, the role of KCC3 in nerve function remains a puzzle. In this study, the microscopic examination of KCC isoforms expressed in peripheral nerves showed high expression of KCC2-4 in nodal segments of the axons and in the perinucleus and microvilli of Schwann cells. The KCC inhibitor [[(dihydroindenyl)oxy]alkanoic acid] but not the Na +-K+-2Cl--cotransport inhibitor (bumetanide) dose-dependently suppressed the amplitude and area of compound muscle action potential, indicating the involvement of KCC activity in peripheral nerve conduction. Furthermore, the amplitude and area under the curve were smaller, and the nerve conduction velocity was slower in nerves from KCC3-/- mice than in nerves from wild-type mice, while the expression pattern of KCC2 and KCC4 was similar in KCC3 kockout and wild-type strains. KCC3-/- mice also manifested a prominent motor deficit in the beam-walking test. This is the first study to demonstrate that the K+-Cl- cotransporter activity of KCC3 contributes to the propagation of action potentials along peripheral nerves.

AB - Nerve conduction requires the fine tuning of ionic currents through delicate interactions between axons and Schwann cells. The K+-Cl - cotransporter (KCC) family includes four isoforms (KCC1-4) that play an important role in the maintenance of cellular osmotic homeostasis via the coupled electroneutral movement of K+ and Cl- with concurrent water flux. Mutation in SLC12A6 gene encoding KCC3 results in an autosomal recessive disease, known as agenesis of the corpus callosum associated with peripheral neuropathy. Nevertheless, the role of KCC3 in nerve function remains a puzzle. In this study, the microscopic examination of KCC isoforms expressed in peripheral nerves showed high expression of KCC2-4 in nodal segments of the axons and in the perinucleus and microvilli of Schwann cells. The KCC inhibitor [[(dihydroindenyl)oxy]alkanoic acid] but not the Na +-K+-2Cl--cotransport inhibitor (bumetanide) dose-dependently suppressed the amplitude and area of compound muscle action potential, indicating the involvement of KCC activity in peripheral nerve conduction. Furthermore, the amplitude and area under the curve were smaller, and the nerve conduction velocity was slower in nerves from KCC3-/- mice than in nerves from wild-type mice, while the expression pattern of KCC2 and KCC4 was similar in KCC3 kockout and wild-type strains. KCC3-/- mice also manifested a prominent motor deficit in the beam-walking test. This is the first study to demonstrate that the K+-Cl- cotransporter activity of KCC3 contributes to the propagation of action potentials along peripheral nerves.

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