Degradation of chitin and production of bioactive materials by bioconversion of squid pens

San Lang Wang, Chiann Wen Yang, Tzu Wen Liang, Juo Hua Peng, Chuan Lu Wang

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Serratia marcescens TKU011, a protease- and chitosanase-producing bacterium, the optimized condition for protease and chitosanase production was found after the media were heated at 121 °C for 120 min and the culture was shaken at 25 °C for 5 days in 100 mL of medium containing 1% squid pen powder (SPP) (w/v), 0.1% K2HPO4, and 0.05% MgSO4. An extracellular metalloprotease with novel properties of solvent stable, and alkaline was purified from the culture supernatant of S. marcescens TKU011 with squid pen wastes as the sole carbon/nitrogen source. The enzyme was a monomeric protease with a molecular mass of 48-50 kDa by SDS-PAGE and gel filtration chromatography. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU011 protease were 8, 50 °C, pH 5-11, and <40 °C, respectively. Besides protease and chitosanase, with this method, deproteinization of squid pen for β-chitin, the production of peptide and reducing sugar may be useful for biological applications.

Original languageEnglish
Pages (from-to)205-212
Number of pages8
JournalCarbohydrate Polymers
Issue number2
Publication statusPublished - 2009 Sep 5

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Degradation of chitin and production of bioactive materials by bioconversion of squid pens'. Together they form a unique fingerprint.

Cite this