Abstract
Tendinopathy of the long head of the biceps (TLHB) involves various types of extracellular matrix degeneration, but previous studies have not evaluated elastic fibers. The purpose of this study was to investigate elastic fiber distribution in long head of the biceps (LHB). The TLHB tendons of 16 consecutive patients (eight men and eight women; average age of 55.75 years; age range of 40–71 years) were transected and harvested. Three cadaveric LHB tendons were used as the control group. The expression of collagen type I was decreased, but type III was increased in TLHB. Disruption of elastic fibers was particularly observed in grade II specimens where the level of elastase-positive staining was significantly higher than in grade I specimens. Elastic fibers were not observed in the grade III area, implying a higher expression of elastase than in the grade I area. Results of Western blotting showed that the expression of elastin was higher in the control group and the levels of elastin significantly decreased in grades II and III of TLHB. Levels of osteopontin and elastase were increased in primary culture of human tenocytes after experiencing elastic derived peptide treatment. These results suggested that elastase may be caused by the disruption of elastic fibers in the development of chronic tendinopathy and that elastic derived peptide may enhance elastase and osteopontin expression.
| Original language | English |
|---|---|
| Pages (from-to) | 1919-1926 |
| Number of pages | 8 |
| Journal | Journal of Orthopaedic Research |
| Volume | 35 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2017 Sep |
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All Science Journal Classification (ASJC) codes
- Orthopedics and Sports Medicine
Cite this
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Degradation of elastic fiber and elevated elastase expression in long head of biceps tendinopathy. / Wu, Yen Ting; Su, Wei Ren; Wu, Po Ting; Shen, Po Chuan; Jou, I. Ming.
In: Journal of Orthopaedic Research, Vol. 35, No. 9, 09.2017, p. 1919-1926.Research output: Contribution to journal › Article
TY - JOUR
T1 - Degradation of elastic fiber and elevated elastase expression in long head of biceps tendinopathy
AU - Wu, Yen Ting
AU - Su, Wei Ren
AU - Wu, Po Ting
AU - Shen, Po Chuan
AU - Jou, I. Ming
PY - 2017/9
Y1 - 2017/9
N2 - Tendinopathy of the long head of the biceps (TLHB) involves various types of extracellular matrix degeneration, but previous studies have not evaluated elastic fibers. The purpose of this study was to investigate elastic fiber distribution in long head of the biceps (LHB). The TLHB tendons of 16 consecutive patients (eight men and eight women; average age of 55.75 years; age range of 40–71 years) were transected and harvested. Three cadaveric LHB tendons were used as the control group. The expression of collagen type I was decreased, but type III was increased in TLHB. Disruption of elastic fibers was particularly observed in grade II specimens where the level of elastase-positive staining was significantly higher than in grade I specimens. Elastic fibers were not observed in the grade III area, implying a higher expression of elastase than in the grade I area. Results of Western blotting showed that the expression of elastin was higher in the control group and the levels of elastin significantly decreased in grades II and III of TLHB. Levels of osteopontin and elastase were increased in primary culture of human tenocytes after experiencing elastic derived peptide treatment. These results suggested that elastase may be caused by the disruption of elastic fibers in the development of chronic tendinopathy and that elastic derived peptide may enhance elastase and osteopontin expression.
AB - Tendinopathy of the long head of the biceps (TLHB) involves various types of extracellular matrix degeneration, but previous studies have not evaluated elastic fibers. The purpose of this study was to investigate elastic fiber distribution in long head of the biceps (LHB). The TLHB tendons of 16 consecutive patients (eight men and eight women; average age of 55.75 years; age range of 40–71 years) were transected and harvested. Three cadaveric LHB tendons were used as the control group. The expression of collagen type I was decreased, but type III was increased in TLHB. Disruption of elastic fibers was particularly observed in grade II specimens where the level of elastase-positive staining was significantly higher than in grade I specimens. Elastic fibers were not observed in the grade III area, implying a higher expression of elastase than in the grade I area. Results of Western blotting showed that the expression of elastin was higher in the control group and the levels of elastin significantly decreased in grades II and III of TLHB. Levels of osteopontin and elastase were increased in primary culture of human tenocytes after experiencing elastic derived peptide treatment. These results suggested that elastase may be caused by the disruption of elastic fibers in the development of chronic tendinopathy and that elastic derived peptide may enhance elastase and osteopontin expression.
UR - http://www.scopus.com/inward/record.url?scp=85016642863&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85016642863&partnerID=8YFLogxK
U2 - 10.1002/jor.23500
DO - 10.1002/jor.23500
M3 - Article
C2 - 27935111
AN - SCOPUS:85016642863
VL - 35
SP - 1919
EP - 1926
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
SN - 0736-0266
IS - 9
ER -