Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata

Yu Wang, He Zhang, Hyok Chol Ri, Zeyu An, Xin Wang, Jia Nan Zhou, Dongran Zheng, Hao Wu, Pengchao Wang, Jianfei Yang, Ding Kun Liu, Diyang Zhang, Wen Chieh Tsai, Zheyong Xue, Zhichao Xu, Peng Zhang, Zhong Jian Liu, Hailong Shen, Yuhua Li

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Araliaceae species produce various classes of triterpene and triterpenoid saponins, such as the oleanane-type triterpenoids in Aralia species and dammarane-type saponins in Panax, valued for their medicinal properties. The lack of genome sequences of Panax relatives has hindered mechanistic insight into the divergence of triterpene saponins in Araliaceae. Here, we report a chromosome-level genome of Aralia elata with a total length of 1.05 Gb. The loss of 12 exons in the dammarenediol synthase (DDS)-encoding gene in A. elata after divergence from Panax might have caused the lack of dammarane-type saponin production, and a complementation assay shows that overexpression of the PgDDS gene from Panax ginseng in callus of A. elata recovers the accumulation of dammarane-type saponins. Tandem duplication events of triterpene biosynthetic genes are common in the A. elata genome, especially for AeCYP72As, AeCSLMs, and AeUGT73s, which function as tailoring enzymes of oleanane-type saponins and aralosides. More than 13 aralosides are de novo synthesized in Saccharomyces cerevisiae by overexpression of these genes in combination. This study sheds light on the diversity of saponins biosynthetic pathway in Araliaceae and will facilitate heterologous bioproduction of aralosides.

Original languageEnglish
Article number2224
JournalNature communications
Volume13
Issue number1
DOIs
Publication statusPublished - 2022 Dec

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • General
  • Physics and Astronomy(all)

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