Dengue virus induces thrombomodulin expression in human endothelial cells and monocytes in vitro

Lien Cheng Chen, Huey Wen Shyu, Hui Min Lin, Huan Yao Lei, Yee Shin Lin, Hsiao Sheng Liu, Trai Ming Yeh

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Objectives: Dengue virus (DV) infections can cause severe life-threatening dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). However, the mechanism to cause hemorrhage in DV infections remains poorly understood. Thrombomodulin (TM), expressed on the surface of endothelial cells and monocytes, is very important in regulation of coagulation and inflammation. Therefore, the effect of DV on the TM expression was studied in vitro using both endothelial cells and monocytes. Methods and results: The expression of TM in human endothelial cell line, HMEC-1, monocytic cell line THP-1 and peripheral blood mononuclear cells derived from human blood was increased after DV infection, UV-inactivated DV or recombinant DV envelop protein domain III stimulation as demonstrated by flow cytometry and immunofluorescent staining. Western blot analysis further confirmed only DV but not enterovirus 71 infection of HMEC-1 cells increased TM protein expression. In addition, RT-PCR analysis showed the increase of TM mRNA as well as other protein C activation-related molecules in DV stimulated HMEC-1 in a dose-dependent manner. Conclusion: These results suggest that DV stimulation of human endothelial cells and monocytes can increase the expression of TM, which may contribute to the anticoagulant properties of cells during DV infection.

Original languageEnglish
Pages (from-to)368-374
Number of pages7
JournalJournal of Infection
Issue number5
Publication statusPublished - 2009 May

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases


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