Dengue virus infection increases microglial cell migration

Ming Kai Jhan, Tsung Ting Tsai, Chia Ling Chen, Cheng Chieh Tsai, Yi Lin Cheng, Yi Chao Lee, Chiung Yuan Ko, Yee-Shin Lin, Chih-Peng Chang, Liang Tzung Lin, Chiou Feng Lin

Research output: Contribution to journalArticle

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Abstract

Activated microglial cells are present in dengue virus (DENV)-infected brains; however, the possible effects of DENV on microglia remain unclear. Here, we demonstrated DENV caused infection, including viral entry, RNA replication, viral protein expression, and virus release, in the murine microglial cell line BV2. DENV infection caused an increase in the formation of the multipolar phenotype in vitro and in vivo without affecting cell growth and cytotoxicity. DENV infection considerably increased cell motility and disrupting either actin filaments or clathrin retarded such effect. Increase in cell migration was only occurred by DENV infection following a clathrin-regulated endocytosis of DENV entry. Ultravioletinactivated DENV did not affect cell migration, and pharmacologically blocking toll-like receptor (TLR) 3 and TLR3-related signaling pathways reduced the DENV-induced increase in cell migration. These results demonstrate an advanced effect of DENV infection on microglial migration via a mechanism involving viral entry, RNA release, and TLR3 signal activation.

Original languageEnglish
Article number91
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Jan 1

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Dengue Virus
Virus Diseases
Cell Movement
Clathrin
Viral RNA
Toll-Like Receptor 3
Virus Release
Virus Internalization
Microglia
Viral Proteins
Endocytosis
Actin Cytoskeleton
Phenotype
Cell Line

All Science Journal Classification (ASJC) codes

  • General

Cite this

Jhan, M. K., Tsai, T. T., Chen, C. L., Tsai, C. C., Cheng, Y. L., Lee, Y. C., ... Lin, C. F. (2017). Dengue virus infection increases microglial cell migration. Scientific reports, 7(1), [91]. https://doi.org/10.1038/s41598-017-00182-z
Jhan, Ming Kai ; Tsai, Tsung Ting ; Chen, Chia Ling ; Tsai, Cheng Chieh ; Cheng, Yi Lin ; Lee, Yi Chao ; Ko, Chiung Yuan ; Lin, Yee-Shin ; Chang, Chih-Peng ; Lin, Liang Tzung ; Lin, Chiou Feng. / Dengue virus infection increases microglial cell migration. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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Jhan, MK, Tsai, TT, Chen, CL, Tsai, CC, Cheng, YL, Lee, YC, Ko, CY, Lin, Y-S, Chang, C-P, Lin, LT & Lin, CF 2017, 'Dengue virus infection increases microglial cell migration', Scientific reports, vol. 7, no. 1, 91. https://doi.org/10.1038/s41598-017-00182-z

Dengue virus infection increases microglial cell migration. / Jhan, Ming Kai; Tsai, Tsung Ting; Chen, Chia Ling; Tsai, Cheng Chieh; Cheng, Yi Lin; Lee, Yi Chao; Ko, Chiung Yuan; Lin, Yee-Shin; Chang, Chih-Peng; Lin, Liang Tzung; Lin, Chiou Feng.

In: Scientific reports, Vol. 7, No. 1, 91, 01.01.2017.

Research output: Contribution to journalArticle

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Jhan MK, Tsai TT, Chen CL, Tsai CC, Cheng YL, Lee YC et al. Dengue virus infection increases microglial cell migration. Scientific reports. 2017 Jan 1;7(1). 91. https://doi.org/10.1038/s41598-017-00182-z