Dengue virus infection induced NF-κB-dependent macrophage migration inhibitory factor production

Lien Cheng Chen, Huey Wen Shyu, Huan Yao Lei, Shun Hua Chen, Hsiao Sheng Liu, Yee Shin Lin, Trai Ming Yeh

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Dengue virus (DV) infection can cause mild dengue fever or severe dengue hemorrhage fever and dengue shock syndrome. Macrophage migration inhibitory factor (MIF) is a cytokine that plays an important role in the modulation of inflammatory and immune responses and serum levels of MIF are correlated with disease severity in dengue patients. However, the mechanism that induces MIF production during DV infection is unclear. In this study, we showed that DV infection, but not UV-inactivated DV stimulation, dose-and time-dependently induced MIF secretion in human A649 epithelial cells. MIF promoter assays and RT-PCR demonstrated that MIF gene transcription was activated during DV infection. Furthermore, DV infection induced NF-κB activation, and the NF-κB inhibitors dexamethasone and curcumin inhibited DV-induced MIF production. Finally, we found that different cells have different abilities to release MIF after DV infection. Interestingly, DV infection and MIF production in the human monocytic cell line THP-1 and peripheral blood mononuclear cells increased in the presence of antibodies against DV. Taken together, these results suggest that DV infection of human cells induces NF-κB activation and MIF production, which can be increased in the presence of pre-existing antibodies.

Original languageEnglish
Pages (from-to)22-31
Number of pages10
JournalAmerican Journal of Infectious Diseases
Volume4
Issue number1
DOIs
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Infectious Diseases

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