Depth positioning for fast moving cells in microflow cytometry utilizing chromatic aberration under a diascopic illuminaiton scheme

Shin Yu Su, Che Hsin Lin, Lung Ming Fu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This research develops a rapid z-position measurement based on the light scattered from the passing particles under a field of light with chromatic aberration. Two band pass filters and two avalanche photodiode (APD) are used for rapid detecting the scattered light from the passing objected. The intensity ratio of two selected band 430 nm-470 nm (blue light) and 630 nm-670 nm (red light) from the scattered light becomes a significant indexes for the depth information of the detecting objects. The throughput of the developed system is capable to for detecting fast moving objects of upto 1000 particles/s which is about 10 folds compared to the spectrometer system. Human erythrocytes is also successfully detected without fluorescence labeling at a high flow velocity of 2.8 mm/s. With this approach, quantitative measurement for the depth position of rapid moving objects inside a sealed microfluidic channel can be achieved in a simple and low cost way.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1825-1827
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015 Jan 1
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country/TerritoryKorea, Republic of
CityGyeongju
Period15-10-2515-10-29

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

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