Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan

Suvendu Das, Sandeep Kar, Jiin Shuh Jean, Jagat Rathod, Sukalyan Chakraborty, Hsiao Sheng Liu, Jochen Bundschuh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The role of arsenite oxidizers in natural attenuation of arsenic pollution necessitates studies on their abundance and diversity in arsenic-contaminated aquifers. In this study, most probable number-polymerase chain reaction (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) was applied to monitor depth-wise abundance and diversity of aerobic arsenite oxidizers in arsenic-enriched groundwater of Beimen, southwestern Taiwan. The results revealed that the abundance of arsenite oxidizers ranged from 0.04 to 0.22, and the lowest ratio was observed in the most arsenic-enriched and comparatively more reduced groundwater (depth 200m) of Beimen 1. The highest ratio was observed in the less arsenic-enriched and less reduced groundwater (depth 60m) of Beimen 2B. DGGE profiles showed a shift in diversity of arsenite oxidizers, consisting of members of the Betaproteobacteria (61%), Alphaproteobacteria (28%) and Gammaproteobacteria (11%), depending on mainly arsenic concentration and redox level in groundwater. Groundwater with the lowest arsenic and highest dissolved oxygen at Beimen 2B harbored 78% of the arsenite oxidizers communities, while groundwater with the highest arsenic and lowest dissolved oxygen at Beimen 1 and Beimen-Jinhu harbored 17 and 22% of arsenite oxidizers communities, respectively. Pseudomonas sp. was found only in groundwater containing high arsenic at Beimen 1 and Beimen-Jinhu, while arsenite oxidizers belonging to Alpha- and Betaproteobacteria were dominated in groundwater containing low arsenic.

Original languageEnglish
Pages (from-to)6983-6991
Number of pages9
JournalWater Research
Volume47
Issue number19
DOIs
Publication statusPublished - 2013 Dec 1

Fingerprint

arsenite
Arsenic
Groundwater
arsenic
Bacteria
bacterium
groundwater
Dissolved oxygen
Electrophoresis
dissolved oxygen
electrokinesis
Gels
gel
Natural attenuation
natural attenuation
Polymerase chain reaction
Aquifers
polymerase chain reaction
Pollution
aquifer

All Science Journal Classification (ASJC) codes

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

Das, Suvendu ; Kar, Sandeep ; Jean, Jiin Shuh ; Rathod, Jagat ; Chakraborty, Sukalyan ; Liu, Hsiao Sheng ; Bundschuh, Jochen. / Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan. In: Water Research. 2013 ; Vol. 47, No. 19. pp. 6983-6991.
@article{2a8ec4160bdc4dac962b1ca3ab67bda4,
title = "Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan",
abstract = "The role of arsenite oxidizers in natural attenuation of arsenic pollution necessitates studies on their abundance and diversity in arsenic-contaminated aquifers. In this study, most probable number-polymerase chain reaction (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) was applied to monitor depth-wise abundance and diversity of aerobic arsenite oxidizers in arsenic-enriched groundwater of Beimen, southwestern Taiwan. The results revealed that the abundance of arsenite oxidizers ranged from 0.04 to 0.22, and the lowest ratio was observed in the most arsenic-enriched and comparatively more reduced groundwater (depth 200m) of Beimen 1. The highest ratio was observed in the less arsenic-enriched and less reduced groundwater (depth 60m) of Beimen 2B. DGGE profiles showed a shift in diversity of arsenite oxidizers, consisting of members of the Betaproteobacteria (61{\%}), Alphaproteobacteria (28{\%}) and Gammaproteobacteria (11{\%}), depending on mainly arsenic concentration and redox level in groundwater. Groundwater with the lowest arsenic and highest dissolved oxygen at Beimen 2B harbored 78{\%} of the arsenite oxidizers communities, while groundwater with the highest arsenic and lowest dissolved oxygen at Beimen 1 and Beimen-Jinhu harbored 17 and 22{\%} of arsenite oxidizers communities, respectively. Pseudomonas sp. was found only in groundwater containing high arsenic at Beimen 1 and Beimen-Jinhu, while arsenite oxidizers belonging to Alpha- and Betaproteobacteria were dominated in groundwater containing low arsenic.",
author = "Suvendu Das and Sandeep Kar and Jean, {Jiin Shuh} and Jagat Rathod and Sukalyan Chakraborty and Liu, {Hsiao Sheng} and Jochen Bundschuh",
year = "2013",
month = "12",
day = "1",
doi = "10.1016/j.watres.2013.07.049",
language = "English",
volume = "47",
pages = "6983--6991",
journal = "Water Research",
issn = "0043-1354",
publisher = "Elsevier Limited",
number = "19",

}

Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan. / Das, Suvendu; Kar, Sandeep; Jean, Jiin Shuh; Rathod, Jagat; Chakraborty, Sukalyan; Liu, Hsiao Sheng; Bundschuh, Jochen.

In: Water Research, Vol. 47, No. 19, 01.12.2013, p. 6983-6991.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Depth-resolved abundance and diversity of arsenite-oxidizing bacteria in the groundwater of Beimen, a blackfoot disease endemic area of southwestern Taiwan

AU - Das, Suvendu

AU - Kar, Sandeep

AU - Jean, Jiin Shuh

AU - Rathod, Jagat

AU - Chakraborty, Sukalyan

AU - Liu, Hsiao Sheng

AU - Bundschuh, Jochen

PY - 2013/12/1

Y1 - 2013/12/1

N2 - The role of arsenite oxidizers in natural attenuation of arsenic pollution necessitates studies on their abundance and diversity in arsenic-contaminated aquifers. In this study, most probable number-polymerase chain reaction (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) was applied to monitor depth-wise abundance and diversity of aerobic arsenite oxidizers in arsenic-enriched groundwater of Beimen, southwestern Taiwan. The results revealed that the abundance of arsenite oxidizers ranged from 0.04 to 0.22, and the lowest ratio was observed in the most arsenic-enriched and comparatively more reduced groundwater (depth 200m) of Beimen 1. The highest ratio was observed in the less arsenic-enriched and less reduced groundwater (depth 60m) of Beimen 2B. DGGE profiles showed a shift in diversity of arsenite oxidizers, consisting of members of the Betaproteobacteria (61%), Alphaproteobacteria (28%) and Gammaproteobacteria (11%), depending on mainly arsenic concentration and redox level in groundwater. Groundwater with the lowest arsenic and highest dissolved oxygen at Beimen 2B harbored 78% of the arsenite oxidizers communities, while groundwater with the highest arsenic and lowest dissolved oxygen at Beimen 1 and Beimen-Jinhu harbored 17 and 22% of arsenite oxidizers communities, respectively. Pseudomonas sp. was found only in groundwater containing high arsenic at Beimen 1 and Beimen-Jinhu, while arsenite oxidizers belonging to Alpha- and Betaproteobacteria were dominated in groundwater containing low arsenic.

AB - The role of arsenite oxidizers in natural attenuation of arsenic pollution necessitates studies on their abundance and diversity in arsenic-contaminated aquifers. In this study, most probable number-polymerase chain reaction (MPN-PCR) and denaturing gradient gel electrophoresis (DGGE) was applied to monitor depth-wise abundance and diversity of aerobic arsenite oxidizers in arsenic-enriched groundwater of Beimen, southwestern Taiwan. The results revealed that the abundance of arsenite oxidizers ranged from 0.04 to 0.22, and the lowest ratio was observed in the most arsenic-enriched and comparatively more reduced groundwater (depth 200m) of Beimen 1. The highest ratio was observed in the less arsenic-enriched and less reduced groundwater (depth 60m) of Beimen 2B. DGGE profiles showed a shift in diversity of arsenite oxidizers, consisting of members of the Betaproteobacteria (61%), Alphaproteobacteria (28%) and Gammaproteobacteria (11%), depending on mainly arsenic concentration and redox level in groundwater. Groundwater with the lowest arsenic and highest dissolved oxygen at Beimen 2B harbored 78% of the arsenite oxidizers communities, while groundwater with the highest arsenic and lowest dissolved oxygen at Beimen 1 and Beimen-Jinhu harbored 17 and 22% of arsenite oxidizers communities, respectively. Pseudomonas sp. was found only in groundwater containing high arsenic at Beimen 1 and Beimen-Jinhu, while arsenite oxidizers belonging to Alpha- and Betaproteobacteria were dominated in groundwater containing low arsenic.

UR - http://www.scopus.com/inward/record.url?scp=84888133413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888133413&partnerID=8YFLogxK

U2 - 10.1016/j.watres.2013.07.049

DO - 10.1016/j.watres.2013.07.049

M3 - Article

C2 - 24169515

AN - SCOPUS:84888133413

VL - 47

SP - 6983

EP - 6991

JO - Water Research

JF - Water Research

SN - 0043-1354

IS - 19

ER -