Abstract
We have developed a swift and simplistic protein immunoassay using aptamer functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique design of the sensor facilitates protein detection in a physiological salt environment overcoming charge screening effects, without requiring sample preprocessing. This study reports a tunable and amplified sensitivity of solution-gated electric double layer (EDL) HEMT-based biosensors, which demonstrates significantly enhanced sensitivity by designing a smaller gap between the gate electrode and the detection, and by operating at higher gate voltage. Sensitivity is calculated by quantifying NT-proBNP, a clinical biomarker of heart failure, in buffer and untreated human serum samples. The biosensor depicts elevated sensitivity and high selectivity. Furthermore, detailed investigation of the amplified sensitivity in an increased ionic strength environment is conducted, and it is revealed that a high sensitivity of 80.54 mV/decade protein concentration can be achieved, which is much higher than that of previously reported FET biosensors. This sensor technology demonstrates immense potential in developing surface affinity sensors for clinical diagnostics.
| Original language | English |
|---|---|
| Pages (from-to) | 5953-5960 |
| Number of pages | 8 |
| Journal | Analytical chemistry |
| Volume | 91 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2019 May 7 |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Design and Demonstration of Tunable Amplified Sensitivity of AlGaN/GaN High Electron Mobility Transistor (HEMT)-Based Biosensors in Human Serum. / Tai, Tse Yu; Sinha, Anirban; Sarangadharan, Indu; Pulikkathodi, Anil Kumar; Wang, Shin Li; Lee, Geng Yen; Chyi, Jen Inn; Shiesh, Shu-Chu; Lee, Gwo Bin; Wang, Yu Lin.
In: Analytical chemistry, Vol. 91, No. 9, 07.05.2019, p. 5953-5960.Research output: Contribution to journal › Article
TY - JOUR
T1 - Design and Demonstration of Tunable Amplified Sensitivity of AlGaN/GaN High Electron Mobility Transistor (HEMT)-Based Biosensors in Human Serum
AU - Tai, Tse Yu
AU - Sinha, Anirban
AU - Sarangadharan, Indu
AU - Pulikkathodi, Anil Kumar
AU - Wang, Shin Li
AU - Lee, Geng Yen
AU - Chyi, Jen Inn
AU - Shiesh, Shu-Chu
AU - Lee, Gwo Bin
AU - Wang, Yu Lin
PY - 2019/5/7
Y1 - 2019/5/7
N2 - We have developed a swift and simplistic protein immunoassay using aptamer functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique design of the sensor facilitates protein detection in a physiological salt environment overcoming charge screening effects, without requiring sample preprocessing. This study reports a tunable and amplified sensitivity of solution-gated electric double layer (EDL) HEMT-based biosensors, which demonstrates significantly enhanced sensitivity by designing a smaller gap between the gate electrode and the detection, and by operating at higher gate voltage. Sensitivity is calculated by quantifying NT-proBNP, a clinical biomarker of heart failure, in buffer and untreated human serum samples. The biosensor depicts elevated sensitivity and high selectivity. Furthermore, detailed investigation of the amplified sensitivity in an increased ionic strength environment is conducted, and it is revealed that a high sensitivity of 80.54 mV/decade protein concentration can be achieved, which is much higher than that of previously reported FET biosensors. This sensor technology demonstrates immense potential in developing surface affinity sensors for clinical diagnostics.
AB - We have developed a swift and simplistic protein immunoassay using aptamer functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique design of the sensor facilitates protein detection in a physiological salt environment overcoming charge screening effects, without requiring sample preprocessing. This study reports a tunable and amplified sensitivity of solution-gated electric double layer (EDL) HEMT-based biosensors, which demonstrates significantly enhanced sensitivity by designing a smaller gap between the gate electrode and the detection, and by operating at higher gate voltage. Sensitivity is calculated by quantifying NT-proBNP, a clinical biomarker of heart failure, in buffer and untreated human serum samples. The biosensor depicts elevated sensitivity and high selectivity. Furthermore, detailed investigation of the amplified sensitivity in an increased ionic strength environment is conducted, and it is revealed that a high sensitivity of 80.54 mV/decade protein concentration can be achieved, which is much higher than that of previously reported FET biosensors. This sensor technology demonstrates immense potential in developing surface affinity sensors for clinical diagnostics.
UR - http://www.scopus.com/inward/record.url?scp=85065487552&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065487552&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.9b00353
DO - 10.1021/acs.analchem.9b00353
M3 - Article
C2 - 30994326
AN - SCOPUS:85065487552
VL - 91
SP - 5953
EP - 5960
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 9
ER -