Design and fabrication of coplanar waveguide probes for single-cell impedance measurement using radio frequency

Chia-Feng Liu, Ming Kun Chen, Ling Sheng Jang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

When cell impedance measurements are conducted at low frequency, the current passes through the outside wall of the cell membrane. The capacitance effect of the double layer and the position of the cell change the impedance. Therefore the pathological change inside the cell cannot be investigated. To overcome the shortcomings of low-frequency measurements, this Letter proposes a method that uses a dielectrophoretic (DEP) trapping technique to position single cells and a coplanar waveguide electrode to measure the impedance of a single HeLa cell (human cervical epithelioid carcinoma) in the frequency range of 1MHz-1GHz. Two materials (a double-sided printed circuit board and glass) are used to fabricate the measurement microelectrodes. Trapping microelectrodes are deposited on the glass substrate near the measurement microelectrodes to trap single cells using the DEP force. The electrical characterisation of a single cell is demonstrated using a two-port vector network analyser. The electrical equivalent model used in this work is validated through measurements of cell impedance in phosphate buffer solution and through electrical simulations of a single HeLa cell.

Original languageEnglish
Pages (from-to)503-509
Number of pages7
JournalMicro and Nano Letters
Volume6
Issue number7
DOIs
Publication statusPublished - 2011 Jul 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

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