Design and numerical analysis of a joule-heating-induced continuous-flow polymerase chain reaction microchip

Qiaole Zhao, Qing An Huang, Yu-Cheng Lin

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We reports a detailed study on a novel design of a Jouleheating-induced polymerase chain reaction (PCR) microchip directly heated by applying electric current to the ends of the geometric varied microchannel, which Is simulated together with the factors acting on the temperature distribution. Treating the continuous flowing fluid in the channel as the resistance, different channel shapes would lead to the different resistances, which generates various heat when the fixed current is applied to the ends of channel. Based on the Ohm's law and the Joule-Thomson effect, three required sequential temperature zones are obtained. The temperature cycling is achieved and the factors that contribute to the temperature distribution are simulated and analyzed, such as the environment temperature, the heat transfer coefficient, the current density, the fluid velocity, and the channel shape. The design of the Joule-heating-induced PCR microchip sheds light on the future novel application of continuous-flow PCR. By applying certain conditions, thermal cycles that meat PCR requiremennts can be achieved.

Original languageEnglish
Article number021102
JournalJournal of Micro/Nanolithography, MEMS, and MOEMS
Volume8
Issue number2
DOIs
Publication statusPublished - 2009 Jan 1

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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