Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli

Shih I. Tan; I. Son Ng

doi:10.1016/j.biortech.2020.123785

Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli

Shih I. Tan
, I. Son Ng

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Global warming is a surging issue that has provoked the demand of green process to mitigate carbon dioxide. In this context, RuBisCo-equipped Escherichia coli has first developed and evaluated the CO₂-assimiliable capability based on the mass balance in three devices: Flask-based in CO₂ incubator (FIC), two-layered device (TLD) and CO₂ bubbling device (CBD) systematically. With the forced diffusion of 5% CO₂ in CBD, which confers an efficient attack of CO₂ to RuBisCo, the CO₂ assimilation increased from −5.03 to −2.63 g-CO₂/g-DCW. Furthermore, boosted CO₂ assimilation ability was observed by co-expression of GroELS chaperone with 71% reduction on CO₂ release. By DNA sequencing and tandem MS/MS analysis, the toxicity of RuBisCo and PRK was identified to interfere the sugar metabolism and energy producing, while the cell morphology was changed and observed in RuBisCo-equipped E. coli. Our study provides a new perspective of higher CO₂ assimilation for sustainable to eco-friendly green bioprocess.

Original language	English
Article number	123785
Journal	Bioresource technology
Volume	314
DOIs	https://doi.org/10.1016/j.biortech.2020.123785
Publication status	Published - 2020 Oct

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy
SDG 13 Climate Action

All Science Journal Classification (ASJC) codes

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2020.123785

Cite this

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title = "Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli",

abstract = "Global warming is a surging issue that has provoked the demand of green process to mitigate carbon dioxide. In this context, RuBisCo-equipped Escherichia coli has first developed and evaluated the CO2-assimiliable capability based on the mass balance in three devices: Flask-based in CO2 incubator (FIC), two-layered device (TLD) and CO2 bubbling device (CBD) systematically. With the forced diffusion of 5\% CO2 in CBD, which confers an efficient attack of CO2 to RuBisCo, the CO2 assimilation increased from −5.03 to −2.63 g-CO2/g-DCW. Furthermore, boosted CO2 assimilation ability was observed by co-expression of GroELS chaperone with 71\% reduction on CO2 release. By DNA sequencing and tandem MS/MS analysis, the toxicity of RuBisCo and PRK was identified to interfere the sugar metabolism and energy producing, while the cell morphology was changed and observed in RuBisCo-equipped E. coli. Our study provides a new perspective of higher CO2 assimilation for sustainable to eco-friendly green bioprocess.",

author = "Tan, \{Shih I.\} and Ng, \{I. Son\}",

note = "Funding Information: The authors are grateful to the financial support for this study provided by the Ministry of Science and Technology (MOST 108-2621-M-006-015, MOST 108-2221-E-006-004-MY3 and MOST 108-2218-E-006-006) in Taiwan. Funding Information: The authors are grateful to the financial support for this study provided by the Ministry of Science and Technology (MOST 108-2621-M-006-015, MOST 108-2221-E-006-004-MY3 and MOST 108-2218-E-006-006) in Taiwan. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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AU - Tan, Shih I.

AU - Ng, I. Son

N1 - Funding Information: The authors are grateful to the financial support for this study provided by the Ministry of Science and Technology (MOST 108-2621-M-006-015, MOST 108-2221-E-006-004-MY3 and MOST 108-2218-E-006-006) in Taiwan. Funding Information: The authors are grateful to the financial support for this study provided by the Ministry of Science and Technology (MOST 108-2621-M-006-015, MOST 108-2221-E-006-004-MY3 and MOST 108-2218-E-006-006) in Taiwan. Publisher Copyright: © 2020 Elsevier Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/10

Y1 - 2020/10

N2 - Global warming is a surging issue that has provoked the demand of green process to mitigate carbon dioxide. In this context, RuBisCo-equipped Escherichia coli has first developed and evaluated the CO2-assimiliable capability based on the mass balance in three devices: Flask-based in CO2 incubator (FIC), two-layered device (TLD) and CO2 bubbling device (CBD) systematically. With the forced diffusion of 5% CO2 in CBD, which confers an efficient attack of CO2 to RuBisCo, the CO2 assimilation increased from −5.03 to −2.63 g-CO2/g-DCW. Furthermore, boosted CO2 assimilation ability was observed by co-expression of GroELS chaperone with 71% reduction on CO2 release. By DNA sequencing and tandem MS/MS analysis, the toxicity of RuBisCo and PRK was identified to interfere the sugar metabolism and energy producing, while the cell morphology was changed and observed in RuBisCo-equipped E. coli. Our study provides a new perspective of higher CO2 assimilation for sustainable to eco-friendly green bioprocess.

AB - Global warming is a surging issue that has provoked the demand of green process to mitigate carbon dioxide. In this context, RuBisCo-equipped Escherichia coli has first developed and evaluated the CO2-assimiliable capability based on the mass balance in three devices: Flask-based in CO2 incubator (FIC), two-layered device (TLD) and CO2 bubbling device (CBD) systematically. With the forced diffusion of 5% CO2 in CBD, which confers an efficient attack of CO2 to RuBisCo, the CO2 assimilation increased from −5.03 to −2.63 g-CO2/g-DCW. Furthermore, boosted CO2 assimilation ability was observed by co-expression of GroELS chaperone with 71% reduction on CO2 release. By DNA sequencing and tandem MS/MS analysis, the toxicity of RuBisCo and PRK was identified to interfere the sugar metabolism and energy producing, while the cell morphology was changed and observed in RuBisCo-equipped E. coli. Our study provides a new perspective of higher CO2 assimilation for sustainable to eco-friendly green bioprocess.

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Design and optimization of bioreactor to boost carbon dioxide assimilation in RuBisCo-equipped Escherichia coli

Abstract

UN SDGs

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