Design of high-resolution analysis technique for capillary electrophoresis microchip

Jik Chang Leong, Chien Hsiung Tsai, Lung-Ming Fu

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In this study, we develop an electrophoresis microfluidic device featuring a conventional cross-form injection system and an expansion chamber located at the inlet of the separation channel. The combined injection system/expansion chamber arrangement is designed to deliver a high-quality sample band into the separation channel such that the detection performance of the device is enhanced. The current microfluidic device is investigated using a sample of Rhodamine B dye. Also, the effects of the expansion chamber on capillary electrophoresis (CE) separation are studied by considering the separation of Hae III-digested φx-174 DNA samples. The results indicate that an expansion chamber with an expansion ratio of 2.5 and an expansion length of 500 μm delivers a sample plug with the correct shape and orientation. The microfluidic device developed in this study has an exciting potential for use in high-performance, high-throughput chemical analysis applications and in many other applications throughout the field of micro-total-analysis systems.

Original languageEnglish
Pages (from-to)6865-6870
Number of pages6
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume46
Issue number10 A
DOIs
Publication statusPublished - 2007 Oct 9

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

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