Desmoplastic tumour-associated stroma versus neural tissue in central nervous system metastasis: Effects of different microenvironments on tumour growth

Kung-Chao Chang, Yin Che Lu, Mei Jung Lin, Hui Yi Chen, Ying Tai Jin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Interactions between tumour cells and extracellular matrix (ECM) are critical in the metastatic cascade. We compared effects of desmoplastic stroma versus neural tissue on central nervous system (CNS) metastasis. Methods and results: Using integrins (ECM receptors), ECM (fibronectin, laminin and collagen IV) and CD31 and vascular endothelial growth factor (VEGF) for angiogenesis, this study examined immunohistochemically 69 consecutive cases of CNS metastases. In contrast to low-level expression in tumour-embedded neural tissue, ECM [fibronectin (71%), laminin γ-1 (79%) and collagen IV (92%)] and CD31-positive microvascular densities (33 versus 4 vessels/field) were significantly richer in desmoplastic tumour stroma, which was present in 90% (53 of 59) of carcinomas, 100% (five of five) of malignant melanomas and 100% (two of two) of sarcomas. Collagen IV expression in tumour stroma was correlated with the expression of fibronectin (P=0.013) and laminin (P=0.034) and with infiltrative tumour edges (P=0.005); fibronectin-positive tumour stroma was correlated with a higher microvascular density (P=0.015). In addition, tumour cells expressed integrins (∼75%) and laminin (84%) more frequently than VEGF (23%), and tumour expression of laminin was correlated with the presence of desmoplastic stroma (P=0.006). Interestingly, laminin-positive tumour stroma was a worse prognosticator (P=0.072). Conclusions: ECM- and vascular-rich stroma is important in tumour growth, which underlies therapeutic strategies targeting tumour-associated stroma.

Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalHistopathology
Volume59
Issue number1
DOIs
Publication statusPublished - 2011 Jul 1

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Tumor Microenvironment
Central Nervous System
Neoplasm Metastasis
Growth
Laminin
Neoplasms
Fibronectins
Extracellular Matrix
Collagen
Integrins
Vascular Endothelial Growth Factor A
Sarcoma
Blood Vessels
Melanoma
Carcinoma

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

Cite this

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title = "Desmoplastic tumour-associated stroma versus neural tissue in central nervous system metastasis: Effects of different microenvironments on tumour growth",
abstract = "Interactions between tumour cells and extracellular matrix (ECM) are critical in the metastatic cascade. We compared effects of desmoplastic stroma versus neural tissue on central nervous system (CNS) metastasis. Methods and results: Using integrins (ECM receptors), ECM (fibronectin, laminin and collagen IV) and CD31 and vascular endothelial growth factor (VEGF) for angiogenesis, this study examined immunohistochemically 69 consecutive cases of CNS metastases. In contrast to low-level expression in tumour-embedded neural tissue, ECM [fibronectin (71{\%}), laminin γ-1 (79{\%}) and collagen IV (92{\%})] and CD31-positive microvascular densities (33 versus 4 vessels/field) were significantly richer in desmoplastic tumour stroma, which was present in 90{\%} (53 of 59) of carcinomas, 100{\%} (five of five) of malignant melanomas and 100{\%} (two of two) of sarcomas. Collagen IV expression in tumour stroma was correlated with the expression of fibronectin (P=0.013) and laminin (P=0.034) and with infiltrative tumour edges (P=0.005); fibronectin-positive tumour stroma was correlated with a higher microvascular density (P=0.015). In addition, tumour cells expressed integrins (∼75{\%}) and laminin (84{\%}) more frequently than VEGF (23{\%}), and tumour expression of laminin was correlated with the presence of desmoplastic stroma (P=0.006). Interestingly, laminin-positive tumour stroma was a worse prognosticator (P=0.072). Conclusions: ECM- and vascular-rich stroma is important in tumour growth, which underlies therapeutic strategies targeting tumour-associated stroma.",
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Desmoplastic tumour-associated stroma versus neural tissue in central nervous system metastasis : Effects of different microenvironments on tumour growth. / Chang, Kung-Chao; Lu, Yin Che; Lin, Mei Jung; Chen, Hui Yi; Jin, Ying Tai.

In: Histopathology, Vol. 59, No. 1, 01.07.2011, p. 31-39.

Research output: Contribution to journalArticle

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