Detecting clonal rearrangement in non-Hodgkin's lymphomas in Taiwan by polymerase chain reaction

Shih Sung Chuang, Ching Nan Lin, Fan Ching Shen, Pei Ju Liao, Yung Liang Liao, Julia Hueimei Chang, Yi Chang Tsai, Chi Yi Cho, Wenya Huang

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The detection of monoclonal expansions of the immunoglobulin heavy chain (IgH) or the T-cell receptor-γ (TCRγ) chain genes is an important supplement for the diagnosis of the non-Hodgkin's lymphomas (NHLs). Detection of monoclonality by polymerase chain reaction (PCR) method has offered an efficient approach for rapid diagnosis and monitoring of the therapeutic effects. Here we conducted a retrospective PCR clonality study on 49 cases of NHLs including 23 B-cell lymphomas (BCLs), 20 peripheral T-cell lymphomas (PTCLs), 6 natural killer (NK)/T-cell lymphomas and 3 reactive lymphoid tissues from southern Taiwan. Genomic DNAs from paraffin sections were extracted and analyzed by the IgH- and TCR-specific PCR reactions. The results showed that 20 of 23 (87.5%) BCLs exhibited IgH gene rearrangements and were all germline for TCRγ. 15 of 20 (75.0%) PTCLs exhibited TCRγ gene rearrangements while 1 case (5%) was positive for IgH gene rearrangement. The 6 NK/T-cell lymphomas and 3 reactive lymphoid tissues were all germline for either IgH or TCRγ genes. Our results were similar to other Western reports in terms of sensitivity and cell-lineage specificity. This is the first large series of PCR clonality study of IgH and TCRγ gene rearrangements on NHLs from Taiwan. We have confirmed that this rapid method is a sensitive diagnostic tool for NHLs.

Original languageEnglish
Pages (from-to)117-121
Number of pages5
JournalLeukemia and Lymphoma
Volume44
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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