Detection and Characterization of Catechol Quinone-Derived Protein Adducts Using Biomolecular Mass Spectrometry

Shu Hui Chen, Chun Wei Li

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

The catechol quinone (CQ) motif is present in many biologically relevant molecules throughout endogenous metabolic products, foods, drugs, and environmental pollutants. The CQ derivatives may undergo Michael addition, and has been shown to yield covalent bonds with nucleophilic sites of cysteine, lysine, or histidine residue of proteins. The CQ-adducted proteins may exhibit cytotoxicity or biological functions different from their un-adducted forms. Identification, characterization, and quantification of relevant protein targets are essential but challenging goals. Mass spectrometry (MS) is well-suited for the analysis of proteins and protein modifications. Technical development of bottom-up proteomics has greatly advanced the field of biomolecular MS, including protein adductomics. This mini-review focuses on the use of biomolecular MS in (1) structural and functional characterization of CQ adduction on standards of proteins, (2) identification of endogenous adduction targets, and (3) quantification of adducted blood proteins as exposure index. The reactivity and outcome of CQ adduction are discussed with emphases on endogenous species, such as dopamine and catechol estrogens. Limitations and advancements in sample preparation, MS instrumentation, and software to facilitate protein adductomics are also discussed.

Original languageEnglish
Article number571
JournalFrontiers in Chemistry
Volume7
DOIs
Publication statusPublished - 2019 Aug 21

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Mass spectrometry
Proteins
Catechol Estrogens
Environmental Pollutants
Covalent bonds
benzoquinone
catechol
Cytotoxicity
Histidine
Lysine
Cysteine
Blood Proteins
Dopamine
Derivatives
Molecules
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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Detection and Characterization of Catechol Quinone-Derived Protein Adducts Using Biomolecular Mass Spectrometry. / Chen, Shu Hui; Li, Chun Wei.

In: Frontiers in Chemistry, Vol. 7, 571, 21.08.2019.

Research output: Contribution to journalReview article

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