Detection and species identification of Cryptosporidium from Taiwan feeding animals

Bing M. Hsu; Hau Yang Wun; Chih-Li Hsu

doi:10.1645/GE-1135.1

Detection and species identification of Cryptosporidium from Taiwan feeding animals

Bing M. Hsu, Hau Yang Wun, Chih-Li Hsu

Department of Parasitology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Cryptosporidium spp. oocysts. Ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of Cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. Nucleic acid sequencing of the 18S rRNA gene identified 3 clusters of Cryptosporidium spp. Three Cryptosporidium parvum isolates were from cattle and sheep feces. One Cryptosporidium andersoni isolate was detected from pig feces. The other 2 novel Cryptosporidium genotypes were not similar to any known Cryptosporidium spp. according to the DNA sequences of the 18S rRNA gene.

Original language	English
Pages (from-to)	252-256
Number of pages	5
Journal	Journal of Parasitology
Volume	94
Issue number	1
DOIs	https://doi.org/10.1645/GE-1135.1
Publication status	Published - 2008 Feb 1

All Science Journal Classification (ASJC) codes

General Agricultural and Biological Sciences
Microbiology
Parasitology

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AB - In this study, 107 fecal specimens were collected from 40 sampling sites in Taiwan livestock and avian farms to test for Cryptosporidium spp. oocysts. Ten of 107 samples analyzed by enzyme-linked immunosorbent assay showed the presence of Cryptosporidium spp., among which 6 samples were simultaneously confirmed by immunofluorescence assay and polymerase chain reaction. Nucleic acid sequencing of the 18S rRNA gene identified 3 clusters of Cryptosporidium spp. Three Cryptosporidium parvum isolates were from cattle and sheep feces. One Cryptosporidium andersoni isolate was detected from pig feces. The other 2 novel Cryptosporidium genotypes were not similar to any known Cryptosporidium spp. according to the DNA sequences of the 18S rRNA gene.

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Detection and species identification of Cryptosporidium from Taiwan feeding animals

Abstract

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