Detection of C-reactive protein on an integrated microfluidic system by utilizing field-effect transistors and aptamers

Wei Chieh Kao, Yen Wen Chen, Chia Ho Chu, Wen Hsin Chang, Shu Chu Shiesh, Yu Lin Wang, Gwo Bin Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cardiovascular diseases (CVDs) cause more than 17 × 106 deaths worldwide on a yearly basis. Early diagnosis of CVDs is therefore of great need. The C-reactive protein (CRP) is an important biomarker for analyzing the risks of CVDs. In this work, CRP-specific aptamers with high sensitivity and specificity and fieldeffect- transistor (FET) devices were used to recognize and detect CRP by using an integrated microfluidic system automatically while consuming less volumes of reagents and samples (about 5 μm). In order to package the FET device into the microfluidic chip, a new method to prevent liquid leakage was proposed. Sensitive detection of CRP has been demonstrated on the developed microfluidic system. It is the first time that aptamer-FET assays could be realized on an integrated microfluidic system. Experimental results showed that the aptamer-FET assay was capable of detecting CRP with concentrations ranging from 0.625 mg/l to 10.000 mg/l, which may be promising for early diagnosis of CVDs.

Original languageEnglish
Article number044105
JournalBiomicrofluidics
Volume11
Issue number4
DOIs
Publication statusPublished - 2017 Jul 1

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Fluid Flow and Transfer Processes
  • Colloid and Surface Chemistry

Cite this