Detection of Differences in mRNA Expression Regulated by Salt-Stress in Mangrove Cultured Cells

Akiyo Yamada, Takeo Saitoh, Yoshihiro Ozeki, Tetsuro Mimura

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Identifying genes involved in salt-stress responses in mangrove plants is one of the most critical steps in elucidating the salt-resistance mechanisms in these plants. In this study, we screened cDNAs whose mRNA amounts were regulated by salt-stress using cDNA Representational Difference Analysis (cDNA RDA) from suspension-cultured cells of a mangrove, Bruguiera sexangula. Four cDNA fragments whose mRNA amounts were up regulated by salt-stress were isolated. Database searches revealed that these cDNAs encode proteins similar to endo-polygalacturonase, alcohol dehydrogenase, and two putative Arabidopsis proteins. In addition, eight cDNA fragments whose mRNA amounts were down regulated by salt stress were also isolated. Further characterization of these cDNAs will likely be important in elucidating the salt-resistance mechanisms in mangrove plants.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalPlant Biotechnology
Volume19
Issue number2
DOIs
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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