Detection of glycated hemoglobin (HbA 1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device

Ya Chun Chuang, Kung Chieh Lan, Ku Meng Hsieh, Ling-Sheng Jang, Ming Kun Chen

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.

Original languageEnglish
Pages (from-to)1222-1230
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume171-172
DOIs
Publication statusPublished - 2012 Aug 1

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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