Detection of glycated hemoglobin (HbA 1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device

Ya Chun Chuang; Kung Chieh Lan; Ku Meng Hsieh; Ling Sheng Jang; Ming Kun Chen

doi:10.1016/j.snb.2012.06.084

Detection of glycated hemoglobin (HbA _1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device

Ya Chun Chuang, Kung Chieh Lan, Ku Meng Hsieh, Ling Sheng Jang, Ming Kun Chen

Department of Electrical Engineering

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA _1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA _1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA _1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.

Original language	English
Pages (from-to)	1222-1230
Number of pages	9
Journal	Sensors and Actuators, B: Chemical
Volume	171-172
DOIs	https://doi.org/10.1016/j.snb.2012.06.084
Publication status	Published - 2012 Aug

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2012.06.084

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title = "Detection of glycated hemoglobin (HbA 1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device",

abstract = "This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.",

author = "Chuang, {Ya Chun} and Lan, {Kung Chieh} and Hsieh, {Ku Meng} and Jang, {Ling Sheng} and Chen, {Ming Kun}",

note = "Funding Information: This study was supported by NCKU-Delta Joint Project, and made use of shared facilities provided under the Program of Top 100 Universities Advancement funded by the Ministry of Education in Taiwan . The authors would also like to thank the Center for Micro/Nano Science and Technology at National Cheng Kung University, Tainan, Taiwan, for access to equipment throughout the duration of this study and for their general technical support. ",

year = "2012",

month = aug,

doi = "10.1016/j.snb.2012.06.084",

language = "English",

volume = "171-172",

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journal = "Sensors and Actuators B: Chemical",

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AU - Chuang, Ya Chun

AU - Lan, Kung Chieh

AU - Hsieh, Ku Meng

AU - Jang, Ling Sheng

AU - Chen, Ming Kun

N1 - Funding Information: This study was supported by NCKU-Delta Joint Project, and made use of shared facilities provided under the Program of Top 100 Universities Advancement funded by the Ministry of Education in Taiwan . The authors would also like to thank the Center for Micro/Nano Science and Technology at National Cheng Kung University, Tainan, Taiwan, for access to equipment throughout the duration of this study and for their general technical support.

PY - 2012/8

Y1 - 2012/8

N2 - This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.

AB - This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.

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Detection of glycated hemoglobin (HbA _1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device

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