Detection of glycated hemoglobin (HbA 1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device

Ya Chun Chuang, Kung Chieh Lan, Ku Meng Hsieh, Ling-Sheng Jang, Ming Kun Chen

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.

Original languageEnglish
Pages (from-to)1222-1230
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume171-172
DOIs
Publication statusPublished - 2012 Aug 1

Fingerprint

microfluidic devices
Hemoglobin
impedance measurement
Glycosylated Hemoglobin A
hemoglobin
Microfluidics
Electrodes
electrodes
Sensors
impedance
Biomolecules
Self assembled monolayers
Thiophene
sensors
Gold
thiophenes
Acids
analyzers
gold
acids

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

@article{90ba435e2d174e31ac6289bd38661219,
title = "Detection of glycated hemoglobin (HbA 1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device",
abstract = "This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.",
author = "Chuang, {Ya Chun} and Lan, {Kung Chieh} and Hsieh, {Ku Meng} and Ling-Sheng Jang and Chen, {Ming Kun}",
year = "2012",
month = "8",
day = "1",
doi = "10.1016/j.snb.2012.06.084",
language = "English",
volume = "171-172",
pages = "1222--1230",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",

}

Detection of glycated hemoglobin (HbA 1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device. / Chuang, Ya Chun; Lan, Kung Chieh; Hsieh, Ku Meng; Jang, Ling-Sheng; Chen, Ming Kun.

In: Sensors and Actuators, B: Chemical, Vol. 171-172, 01.08.2012, p. 1222-1230.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of glycated hemoglobin (HbA 1c) based on impedance measurement with parallel electrodes integrated into a microfluidic device

AU - Chuang, Ya Chun

AU - Lan, Kung Chieh

AU - Hsieh, Ku Meng

AU - Jang, Ling-Sheng

AU - Chen, Ming Kun

PY - 2012/8/1

Y1 - 2012/8/1

N2 - This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.

AB - This paper proposes a sensor for glycated hemoglobin (HbA1c) detection based on impedance measurement. The sensor contains a pair of parallel facing electrodes integrated into a microfluidic device. A pair of parallel facing electrodes is fabricated to characterize the impedance change and determine the concentration of HbA 1c. A self-assembled monolayer (SAM) of thiophene-3-boronic acid (T3BA) is used for modifying the gold electrodes. Non-faradaic electrochemical detection of surface-bound HbA 1c with no redox indicator added is conducted by utilizing the capacitive property as a sensing signal. The performance of the HbA 1c sensor was investigated by impedance analyzer and the impedance changes approximately logarithmically with the concentration of HbA1c in the range of 10-100 ng/μL. The proposed detection method has potential for use in point-of-care diagnostics and can be used for the detection of various biomolecules.

UR - http://www.scopus.com/inward/record.url?scp=84864288186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864288186&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2012.06.084

DO - 10.1016/j.snb.2012.06.084

M3 - Article

VL - 171-172

SP - 1222

EP - 1230

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

ER -