Detection of hepatitis C virus RNA in peripheral blood mononuclear cells and in saliva

Kung-Chia Young, Ting-Tsung Chang, Tai‐Cherng ‐C Liou, Hua-Lin Wu

Research output: Contribution to journalArticle

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Abstract

The nested polymerase chain reaction (PCR) technique was applied to investigate hepatitis C virus (HCV) RNA in the peripheral blood mononuclear cells (PBMCs), saliva, and serum of patients with chronic type C hepatitis. The specificity of the amplified products was analyzed and confirmed by agarose gel electrophoresis, Southern blot hybridization, and restriction endonuclease pattern analysis. HCV RNA was detectable in the PBMCs of 24% (12/50) of the patients. The HCV RNA detected in PBMCs was not due to the contamination from plasma, since no viral sequences could be detected in the third washing of PBMCs. Of the 12 patients with HCV RNA in PBMCs, five were negative for HCV RNA sequences in the serum. Thus the presence of HCV RNA in PBMCs was not strictly correlated to the results for sera. Among 25 patients with HCV RNA in their saliva, 18 were negative for PBMCs. Among 25 patients without HCV RNA in their saliva, five had HCV RNA in PBMCs. In conclusion, PBMCs are an extrahepatic target for chronic HCV infection. However, we do not suggest that PBMCs act as a vehicle for carrying HCV to saliva, since the presence of HCV RNA in PBMCs and in saliva was not closely correlated.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalJournal of Medical Virology
Volume41
Issue number1
DOIs
Publication statusPublished - 1993 Jan 1

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Saliva
Hepacivirus
Blood Cells
RNA
Chronic Hepatitis C
Serum
Agar Gel Electrophoresis
DNA Restriction Enzymes
Virus Diseases
Southern Blotting
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

Cite this

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title = "Detection of hepatitis C virus RNA in peripheral blood mononuclear cells and in saliva",
abstract = "The nested polymerase chain reaction (PCR) technique was applied to investigate hepatitis C virus (HCV) RNA in the peripheral blood mononuclear cells (PBMCs), saliva, and serum of patients with chronic type C hepatitis. The specificity of the amplified products was analyzed and confirmed by agarose gel electrophoresis, Southern blot hybridization, and restriction endonuclease pattern analysis. HCV RNA was detectable in the PBMCs of 24{\%} (12/50) of the patients. The HCV RNA detected in PBMCs was not due to the contamination from plasma, since no viral sequences could be detected in the third washing of PBMCs. Of the 12 patients with HCV RNA in PBMCs, five were negative for HCV RNA sequences in the serum. Thus the presence of HCV RNA in PBMCs was not strictly correlated to the results for sera. Among 25 patients with HCV RNA in their saliva, 18 were negative for PBMCs. Among 25 patients without HCV RNA in their saliva, five had HCV RNA in PBMCs. In conclusion, PBMCs are an extrahepatic target for chronic HCV infection. However, we do not suggest that PBMCs act as a vehicle for carrying HCV to saliva, since the presence of HCV RNA in PBMCs and in saliva was not closely correlated.",
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Detection of hepatitis C virus RNA in peripheral blood mononuclear cells and in saliva. / Young, Kung-Chia; Chang, Ting-Tsung; Liou, Tai‐Cherng ‐C; Wu, Hua-Lin.

In: Journal of Medical Virology, Vol. 41, No. 1, 01.01.1993, p. 55-60.

Research output: Contribution to journalArticle

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