TY - JOUR
T1 - Detection of live SARS-CoV-2 virus and its variants by specially designed SERS-active substrates and spectroscopic analyses
AU - Sitjar, Jaya
AU - Liao, Jiunn Der
AU - Lee, Han
AU - Tsai, Huey Pin
AU - Wang, Jen Ren
AU - Chen, Chien Hsiung
AU - Wang, Hao
AU - Liu, Bernard Haochih
N1 - Funding Information:
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jiunn-Der Liao reports financial support was provided by National Science Council of Taiwan. Jiunn-Der Liao reports a relationship with National Science Council of Taiwan that includes: funding grants. Jiunn-Der Liao has patent pending to National Cheng Kung University.This work was supported by the National Science Council of Taiwan (grant numbers: NSC 110-2221-E-006 -014 -MY3, 109-2224-E-006-008, 109-2327-B-006-005, 109-2811-E-006-531-MY2, and 111-2321-B-006-009 and National Cheng Kung University Hospital (grant number: NCKUH-11108001). The BSL-3 Virology Laboratory of National Cheng Kung University Hospital, Tainan, Taiwan (with authorization), and Center for Drug Evaluation (CDE) of Taiwan (case number: 109IDX08030). The authors declare no conflict of interest.
Funding Information:
This work was supported by the National Science Council of Taiwan (grant numbers: NSC 110-2221-E-006 -014 -MY3 , 109-2224-E-006-008 , 109-2327-B-006-005 , 109-2811-E-006-531-MY2 , and 111-2321-B-006-009 and National Cheng Kung University Hospital (grant number: NCKUH-11108001 ). The BSL-3 Virology Laboratory of National Cheng Kung University Hospital, Tainan, Taiwan (with authorization), and Center for Drug Evaluation (CDE) of Taiwan (case number: 109IDX08030). The authors declare no conflict of interest.
Publisher Copyright:
© 2023
PY - 2023/5/22
Y1 - 2023/5/22
N2 - A method using label-free surface enhanced Raman spectroscopy (SERS) based on substrate design is provided for an early detection and differentiation of spike glycoprotein mutation sites in live SARS-CoV-2 variants. Two SERS-active substrates, Au nanocavities (Au NCs) and Au NPs on porous ZrO2 (Au NPs/pZrO2), were used to identify specific peaks of A.3, Alpha, and Delta variants at different concentrations and demonstrated the ability to provide their SERS spectra with detection limits of 0.1–1.0% (or 104−5 copies/mL). Variant identification can be achieved by cross-examining reference spectra and analyzing the substrate-analyte relationship between the suitability of the analyte upon the hotspot(s) formed at high concentrations and the effective detection distance at low concentrations. Mutation sites on the S1 chain of the spike glycoprotein for each variant may be related and distinguishable. This method does not require sample preprocessing and therefore allows for fast screening, which is of high value for more comprehensive and specific studies to distinguish upcoming variants.
AB - A method using label-free surface enhanced Raman spectroscopy (SERS) based on substrate design is provided for an early detection and differentiation of spike glycoprotein mutation sites in live SARS-CoV-2 variants. Two SERS-active substrates, Au nanocavities (Au NCs) and Au NPs on porous ZrO2 (Au NPs/pZrO2), were used to identify specific peaks of A.3, Alpha, and Delta variants at different concentrations and demonstrated the ability to provide their SERS spectra with detection limits of 0.1–1.0% (or 104−5 copies/mL). Variant identification can be achieved by cross-examining reference spectra and analyzing the substrate-analyte relationship between the suitability of the analyte upon the hotspot(s) formed at high concentrations and the effective detection distance at low concentrations. Mutation sites on the S1 chain of the spike glycoprotein for each variant may be related and distinguishable. This method does not require sample preprocessing and therefore allows for fast screening, which is of high value for more comprehensive and specific studies to distinguish upcoming variants.
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U2 - 10.1016/j.aca.2023.341151
DO - 10.1016/j.aca.2023.341151
M3 - Article
C2 - 37037632
AN - SCOPUS:85151284895
SN - 0003-2670
VL - 1256
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
M1 - 341151
ER -