TY - JOUR
T1 - Determination of degree of RBC agglutination for blood typing using a small quantity of blood sample in a microfluidic system
AU - Chang, Yaw Jen
AU - Ho, Ching Yuan
AU - Zhou, Xin Miao
AU - Yen, Hsiu Rong
N1 - Funding Information:
This work was partially supported by the Ministry of Science and Technology (MOST) of Taiwan, R.O.C. [Grant number MOST 106-2221-E-033-069 ]
PY - 2018/4/15
Y1 - 2018/4/15
N2 - Blood typing assay is a critical test to ensure the serological compatibility of a donor and an intended recipient prior to a blood transfusion. This paper presents a microfluidic blood typing system using a small quantity of blood sample to determine the degree of agglutination of red blood cell (RBC). Two measuring methods were proposed: impedimetric measurement and electroanalytical measurement. The charge transfer resistance in the impedimetric measurement and the power parameter in the electroanalytical measurement were used for the analysis of agglutination level. From the experimental results, both measuring methods provide quantitative results, and the parameters are linearly and monotonically related to the degree of RBC agglutination. However, the electroanalytical measurement is more reliable than the impedimetric technique because the impedimetric measurement may suffer from many influencing factors, such as chip conditions. Five levels from non-agglutination (level 0) to strong agglutination (level 4+) can be discriminated in this study, conforming to the clinical requirement to prevent any risks in transfusion.
AB - Blood typing assay is a critical test to ensure the serological compatibility of a donor and an intended recipient prior to a blood transfusion. This paper presents a microfluidic blood typing system using a small quantity of blood sample to determine the degree of agglutination of red blood cell (RBC). Two measuring methods were proposed: impedimetric measurement and electroanalytical measurement. The charge transfer resistance in the impedimetric measurement and the power parameter in the electroanalytical measurement were used for the analysis of agglutination level. From the experimental results, both measuring methods provide quantitative results, and the parameters are linearly and monotonically related to the degree of RBC agglutination. However, the electroanalytical measurement is more reliable than the impedimetric technique because the impedimetric measurement may suffer from many influencing factors, such as chip conditions. Five levels from non-agglutination (level 0) to strong agglutination (level 4+) can be discriminated in this study, conforming to the clinical requirement to prevent any risks in transfusion.
UR - http://www.scopus.com/inward/record.url?scp=85034105003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85034105003&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2017.11.008
DO - 10.1016/j.bios.2017.11.008
M3 - Article
C2 - 29153944
AN - SCOPUS:85034105003
SN - 0956-5663
VL - 102
SP - 234
EP - 241
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -