Abstract
Lamotrigine is a broad-spectrum antiepileptic agent. This study describes a simple and sensitive high-performance liquid chromatographic method for the determination of lamotrigine in 50 μl of plasma. Lamotrigine and the internal standard guanabenz were extracted with 1.2 ml of diethyl ether, after the samples alkalinized with 10 μl of sodium hydroxide solution (1N). Chromatographic separation was achieved on a silica column with the mobile phase of acetonitrile-water containing 0.2% phosphoric acid and 0.3% triethylamine (pH 2.7) (84:16, v/v), at a flow-rate of 1 ml/min. The eluant was detected at 225 nm. The retention time was about 6 min for lamotrigine and 7 min for guanabenz. No endogenous substances and concomitant anticonvulsants were found to interfere. Calibration curves were linear from 0.1 to 5 μg/ml. The relative recovery of lamotrigine averaged about 80%. The limit of quantitation was 0.1 μg/ml. The intra- and inter-day precision (expressed as coefficient of variation, CV) was 8.1%, or less, and the accuracy was within 11.5% deviation of the nominal concentration. The method is suitable in pharmacokinetic investigation and monitoring lamotrigine concentration.
| Original language | English |
|---|---|
| Pages (from-to) | 199-206 |
| Number of pages | 8 |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 817 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2005 Mar 25 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biochemistry
Cite this
}
Determination of lamotrigine in small volumes of plasma by high-performance liquid chromatography. / Cheng, Ching Ling; Chou, Chen-Hsi; Hu, Oliver Yoa Pu.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 817, No. 2, 25.03.2005, p. 199-206.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of lamotrigine in small volumes of plasma by high-performance liquid chromatography
AU - Cheng, Ching Ling
AU - Chou, Chen-Hsi
AU - Hu, Oliver Yoa Pu
PY - 2005/3/25
Y1 - 2005/3/25
N2 - Lamotrigine is a broad-spectrum antiepileptic agent. This study describes a simple and sensitive high-performance liquid chromatographic method for the determination of lamotrigine in 50 μl of plasma. Lamotrigine and the internal standard guanabenz were extracted with 1.2 ml of diethyl ether, after the samples alkalinized with 10 μl of sodium hydroxide solution (1N). Chromatographic separation was achieved on a silica column with the mobile phase of acetonitrile-water containing 0.2% phosphoric acid and 0.3% triethylamine (pH 2.7) (84:16, v/v), at a flow-rate of 1 ml/min. The eluant was detected at 225 nm. The retention time was about 6 min for lamotrigine and 7 min for guanabenz. No endogenous substances and concomitant anticonvulsants were found to interfere. Calibration curves were linear from 0.1 to 5 μg/ml. The relative recovery of lamotrigine averaged about 80%. The limit of quantitation was 0.1 μg/ml. The intra- and inter-day precision (expressed as coefficient of variation, CV) was 8.1%, or less, and the accuracy was within 11.5% deviation of the nominal concentration. The method is suitable in pharmacokinetic investigation and monitoring lamotrigine concentration.
AB - Lamotrigine is a broad-spectrum antiepileptic agent. This study describes a simple and sensitive high-performance liquid chromatographic method for the determination of lamotrigine in 50 μl of plasma. Lamotrigine and the internal standard guanabenz were extracted with 1.2 ml of diethyl ether, after the samples alkalinized with 10 μl of sodium hydroxide solution (1N). Chromatographic separation was achieved on a silica column with the mobile phase of acetonitrile-water containing 0.2% phosphoric acid and 0.3% triethylamine (pH 2.7) (84:16, v/v), at a flow-rate of 1 ml/min. The eluant was detected at 225 nm. The retention time was about 6 min for lamotrigine and 7 min for guanabenz. No endogenous substances and concomitant anticonvulsants were found to interfere. Calibration curves were linear from 0.1 to 5 μg/ml. The relative recovery of lamotrigine averaged about 80%. The limit of quantitation was 0.1 μg/ml. The intra- and inter-day precision (expressed as coefficient of variation, CV) was 8.1%, or less, and the accuracy was within 11.5% deviation of the nominal concentration. The method is suitable in pharmacokinetic investigation and monitoring lamotrigine concentration.
UR - http://www.scopus.com/inward/record.url?scp=15944384647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15944384647&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2004.12.004
DO - 10.1016/j.jchromb.2004.12.004
M3 - Article
VL - 817
SP - 199
EP - 206
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 2
ER -