Determination of lamotrigine in small volumes of plasma by high-performance liquid chromatography

Ching Ling Cheng, Chen-Hsi Chou, Oliver Yoa Pu Hu

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Lamotrigine is a broad-spectrum antiepileptic agent. This study describes a simple and sensitive high-performance liquid chromatographic method for the determination of lamotrigine in 50 μl of plasma. Lamotrigine and the internal standard guanabenz were extracted with 1.2 ml of diethyl ether, after the samples alkalinized with 10 μl of sodium hydroxide solution (1N). Chromatographic separation was achieved on a silica column with the mobile phase of acetonitrile-water containing 0.2% phosphoric acid and 0.3% triethylamine (pH 2.7) (84:16, v/v), at a flow-rate of 1 ml/min. The eluant was detected at 225 nm. The retention time was about 6 min for lamotrigine and 7 min for guanabenz. No endogenous substances and concomitant anticonvulsants were found to interfere. Calibration curves were linear from 0.1 to 5 μg/ml. The relative recovery of lamotrigine averaged about 80%. The limit of quantitation was 0.1 μg/ml. The intra- and inter-day precision (expressed as coefficient of variation, CV) was 8.1%, or less, and the accuracy was within 11.5% deviation of the nominal concentration. The method is suitable in pharmacokinetic investigation and monitoring lamotrigine concentration.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume817
Issue number2
DOIs
Publication statusPublished - 2005 Mar 25

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Plasma Volume
High performance liquid chromatography
High Pressure Liquid Chromatography
Plasmas
Guanabenz
Anticonvulsants
Sodium Hydroxide
Pharmacokinetics
Silicon Dioxide
Ether
Calibration
lamotrigine
Flow rate
Recovery
Water
Monitoring
Liquids

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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abstract = "Lamotrigine is a broad-spectrum antiepileptic agent. This study describes a simple and sensitive high-performance liquid chromatographic method for the determination of lamotrigine in 50 μl of plasma. Lamotrigine and the internal standard guanabenz were extracted with 1.2 ml of diethyl ether, after the samples alkalinized with 10 μl of sodium hydroxide solution (1N). Chromatographic separation was achieved on a silica column with the mobile phase of acetonitrile-water containing 0.2{\%} phosphoric acid and 0.3{\%} triethylamine (pH 2.7) (84:16, v/v), at a flow-rate of 1 ml/min. The eluant was detected at 225 nm. The retention time was about 6 min for lamotrigine and 7 min for guanabenz. No endogenous substances and concomitant anticonvulsants were found to interfere. Calibration curves were linear from 0.1 to 5 μg/ml. The relative recovery of lamotrigine averaged about 80{\%}. The limit of quantitation was 0.1 μg/ml. The intra- and inter-day precision (expressed as coefficient of variation, CV) was 8.1{\%}, or less, and the accuracy was within 11.5{\%} deviation of the nominal concentration. The method is suitable in pharmacokinetic investigation and monitoring lamotrigine concentration.",
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Determination of lamotrigine in small volumes of plasma by high-performance liquid chromatography. / Cheng, Ching Ling; Chou, Chen-Hsi; Hu, Oliver Yoa Pu.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 817, No. 2, 25.03.2005, p. 199-206.

Research output: Contribution to journalArticle

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