Determination of vecuronium in blood by HPLC with UV and electrochemical detection

a pilot study in man.

O. Y. Hu, Chen-Hsi Chou, W. Ho, S. T. Ho

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Vecuronium bromide is a non-depolarizing neuromuscular blocking agent with a rather low therapeutic level. Rapid, sensitive, and selective determination of vecuronium bromide in human blood or plasma was essential for pharmacokinetic study. We developed such a method using HPLC with electrochemical detection. Samples were first acidified, followed by a one-step liquid-liquid extraction. Tubocurarine, which has a structure similar to that of vecuronium, was used as the internal standard. The electrochemical detector, Ag/AgCl electrodes, was operated by setting the working electrodes, W1 and W2, at +0.65 V and +1.05 V, respectively. When vecuronium blood concentration was plotted versus peak area ratios (PAR) of vecuronium over tubocurarine, a linear relationship was observed over the range of 25 ng/mL to 500 ng/mL with a correlation coefficient greater than 0.997. Clinically possible sources of interference, such as atropine, apresoline, droperidol, fentanyl, labetalol, thiopentone, atracurium, and valium, were examined and none showed interference in the assay of vecuronium and tubocurarine. This method has been successfully applied in a preliminary study of the pharmacokinetics of vecuronium in a patient undergoing surgery. The low detection limit of this method in the patient was 3 ng/mL.

Original languageEnglish
Pages (from-to)186-190
Number of pages5
JournalProceedings of the National Science Council, Republic of China. Part B, Life sciences
Volume15
Issue number3
Publication statusPublished - 1991 Jan 1

Fingerprint

Vecuronium Bromide
High Pressure Liquid Chromatography
Tubocurarine
Electrodes
Pharmacokinetics
Atracurium
Labetalol
Neuromuscular Blocking Agents
Hydralazine
Liquid-Liquid Extraction
Thiopental
Diazepam
Atropine
Limit of Detection

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{ff4ed3bd31ba49b8a5c9c91df86644d5,
title = "Determination of vecuronium in blood by HPLC with UV and electrochemical detection: a pilot study in man.",
abstract = "Vecuronium bromide is a non-depolarizing neuromuscular blocking agent with a rather low therapeutic level. Rapid, sensitive, and selective determination of vecuronium bromide in human blood or plasma was essential for pharmacokinetic study. We developed such a method using HPLC with electrochemical detection. Samples were first acidified, followed by a one-step liquid-liquid extraction. Tubocurarine, which has a structure similar to that of vecuronium, was used as the internal standard. The electrochemical detector, Ag/AgCl electrodes, was operated by setting the working electrodes, W1 and W2, at +0.65 V and +1.05 V, respectively. When vecuronium blood concentration was plotted versus peak area ratios (PAR) of vecuronium over tubocurarine, a linear relationship was observed over the range of 25 ng/mL to 500 ng/mL with a correlation coefficient greater than 0.997. Clinically possible sources of interference, such as atropine, apresoline, droperidol, fentanyl, labetalol, thiopentone, atracurium, and valium, were examined and none showed interference in the assay of vecuronium and tubocurarine. This method has been successfully applied in a preliminary study of the pharmacokinetics of vecuronium in a patient undergoing surgery. The low detection limit of this method in the patient was 3 ng/mL.",
author = "Hu, {O. Y.} and Chen-Hsi Chou and W. Ho and Ho, {S. T.}",
year = "1991",
month = "1",
day = "1",
language = "English",
volume = "15",
pages = "186--190",
journal = "Proceedings of the National Science Council, Republic of China. Part B, Life sciences",
issn = "0255-6596",
publisher = "National Science Council",
number = "3",

}

TY - JOUR

T1 - Determination of vecuronium in blood by HPLC with UV and electrochemical detection

T2 - a pilot study in man.

AU - Hu, O. Y.

AU - Chou, Chen-Hsi

AU - Ho, W.

AU - Ho, S. T.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Vecuronium bromide is a non-depolarizing neuromuscular blocking agent with a rather low therapeutic level. Rapid, sensitive, and selective determination of vecuronium bromide in human blood or plasma was essential for pharmacokinetic study. We developed such a method using HPLC with electrochemical detection. Samples were first acidified, followed by a one-step liquid-liquid extraction. Tubocurarine, which has a structure similar to that of vecuronium, was used as the internal standard. The electrochemical detector, Ag/AgCl electrodes, was operated by setting the working electrodes, W1 and W2, at +0.65 V and +1.05 V, respectively. When vecuronium blood concentration was plotted versus peak area ratios (PAR) of vecuronium over tubocurarine, a linear relationship was observed over the range of 25 ng/mL to 500 ng/mL with a correlation coefficient greater than 0.997. Clinically possible sources of interference, such as atropine, apresoline, droperidol, fentanyl, labetalol, thiopentone, atracurium, and valium, were examined and none showed interference in the assay of vecuronium and tubocurarine. This method has been successfully applied in a preliminary study of the pharmacokinetics of vecuronium in a patient undergoing surgery. The low detection limit of this method in the patient was 3 ng/mL.

AB - Vecuronium bromide is a non-depolarizing neuromuscular blocking agent with a rather low therapeutic level. Rapid, sensitive, and selective determination of vecuronium bromide in human blood or plasma was essential for pharmacokinetic study. We developed such a method using HPLC with electrochemical detection. Samples were first acidified, followed by a one-step liquid-liquid extraction. Tubocurarine, which has a structure similar to that of vecuronium, was used as the internal standard. The electrochemical detector, Ag/AgCl electrodes, was operated by setting the working electrodes, W1 and W2, at +0.65 V and +1.05 V, respectively. When vecuronium blood concentration was plotted versus peak area ratios (PAR) of vecuronium over tubocurarine, a linear relationship was observed over the range of 25 ng/mL to 500 ng/mL with a correlation coefficient greater than 0.997. Clinically possible sources of interference, such as atropine, apresoline, droperidol, fentanyl, labetalol, thiopentone, atracurium, and valium, were examined and none showed interference in the assay of vecuronium and tubocurarine. This method has been successfully applied in a preliminary study of the pharmacokinetics of vecuronium in a patient undergoing surgery. The low detection limit of this method in the patient was 3 ng/mL.

UR - http://www.scopus.com/inward/record.url?scp=0026185101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026185101&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 186

EP - 190

JO - Proceedings of the National Science Council, Republic of China. Part B, Life sciences

JF - Proceedings of the National Science Council, Republic of China. Part B, Life sciences

SN - 0255-6596

IS - 3

ER -