Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation

Seung Koo Lee; Zhenhua Shen; Myung Shin Han; Ching Hsuan Tung

doi:10.1039/d2ra00377e

Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation

Seung Koo Lee
, Zhenhua Shen
, Myung Shin Han
, Ching Hsuan Tung

Department of Oncology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A methylene blue (MB)-based beta-galactosidase (β-gal) activatable molecule, Gal-MB, was developed for senescence imaging and light-triggered senolysis. When in contact with LacZ β-gal or senescence-associated β-gal (SA-β-gal), the photoinsensitive Gal-MB becomes fluorescent. Gal-MB also offered selective phototoxicity toward LacZ β-gal expressing cells and drug-induced senescent cells, which express SA-β-gal, after light illumination at 665 nm.

Original language	English
Pages (from-to)	4543-4549
Number of pages	7
Journal	RSC Advances
Volume	12
Issue number	8
DOIs	https://doi.org/10.1039/d2ra00377e
Publication status	Published - 2022 Feb 4

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering

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10.1039/d2ra00377e

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title = "Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation",

abstract = "A methylene blue (MB)-based beta-galactosidase (β-gal) activatable molecule, Gal-MB, was developed for senescence imaging and light-triggered senolysis. When in contact with LacZ β-gal or senescence-associated β-gal (SA-β-gal), the photoinsensitive Gal-MB becomes fluorescent. Gal-MB also offered selective phototoxicity toward LacZ β-gal expressing cells and drug-induced senescent cells, which express SA-β-gal, after light illumination at 665 nm.",

author = "Lee, \{Seung Koo\} and Zhenhua Shen and Han, \{Myung Shin\} and Tung, \{Ching Hsuan\}",

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doi = "10.1039/d2ra00377e",

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TY - JOUR

T1 - Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation

AU - Lee, Seung Koo

AU - Shen, Zhenhua

AU - Han, Myung Shin

AU - Tung, Ching Hsuan

N1 - Publisher Copyright: © The Royal Society of Chemistry.

PY - 2022/2/4

Y1 - 2022/2/4

N2 - A methylene blue (MB)-based beta-galactosidase (β-gal) activatable molecule, Gal-MB, was developed for senescence imaging and light-triggered senolysis. When in contact with LacZ β-gal or senescence-associated β-gal (SA-β-gal), the photoinsensitive Gal-MB becomes fluorescent. Gal-MB also offered selective phototoxicity toward LacZ β-gal expressing cells and drug-induced senescent cells, which express SA-β-gal, after light illumination at 665 nm.

AB - A methylene blue (MB)-based beta-galactosidase (β-gal) activatable molecule, Gal-MB, was developed for senescence imaging and light-triggered senolysis. When in contact with LacZ β-gal or senescence-associated β-gal (SA-β-gal), the photoinsensitive Gal-MB becomes fluorescent. Gal-MB also offered selective phototoxicity toward LacZ β-gal expressing cells and drug-induced senescent cells, which express SA-β-gal, after light illumination at 665 nm.

UR - https://www.scopus.com/pages/publications/85124689803

UR - https://www.scopus.com/pages/publications/85124689803#tab=citedBy

U2 - 10.1039/d2ra00377e

DO - 10.1039/d2ra00377e

M3 - Article

AN - SCOPUS:85124689803

SN - 2046-2069

VL - 12

SP - 4543

EP - 4549

JO - RSC Advances

JF - RSC Advances

IS - 8

ER -

Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation

Abstract

All Science Journal Classification (ASJC) codes

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