Development of 12 genic microsatellite loci for a biofuel grass, Miscanthus sinensis (Poaceae)

Chuan Wen Ho, Tai Han Wu, Tsai Wen Hsu, Jao Ching Huang, Chi Chun Huang, Tzen Yuh Chiang

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Premise of the study: Miscanthus, a nonfood plant with high potential as a biofuel, has been used in Europe and the United States. The selection of a cultivar with high biomass, photosynthetic efficiency, and stress resistance from wild populations has become an important issue. New genic microsatellite markers will aid the assessment of genetic diversity for different strains. Methods and Results: Twelve polymorphic microsatellite markers derived from the transcriptome of Miscanthus sinensis fo. glaber were identified and screened on 80 individuals of M. sinensis. The number of alleles per locus ranged from 6 to 12, and the mean expected heterozygosity was 0.75. Cross-taxa transferability revealed that all loci can be applied to all varieties of M. sinensis, as well as the closely related species M. floridulus. Conclusions: These new genic microsatellite markers are useful for characterizing different traits in breeding programs or to select genes useful for biofuel.

Original languageEnglish
Pages (from-to)e201-e203
JournalAmerican Journal of Botany
Volume98
Issue number8
DOIs
Publication statusPublished - 2011 Aug

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

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