Development of a bead-based optoelectrokinetic immunosensing technique for detection of low-abundance analytes

Han-Sheng Chuang, Hsiao Neng Lin, Hu Yao Ku

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bead-based immunosensing has been growing as a promising technology in the point-of-care diagnostics because of great flexibility. For dilute samples, functionalized particles can be used to collect dispersed analytes and act as carriers for particle manipulation. To carry out rapid and selective diagnosis, a bead-based optoelectrokinetic immunosensing technique was developed herein to detect biomarkers, lipocalin 1 (LCN1) and TNF-α, for diabetic retinopathy (DR). The measurement was made in a sample droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam simultaneously applying on the microchip, the immunocomplexes in the droplet were further concentrated within the region of irradiation to enhance the fluorescent signal. The optoelectrokinetic platform, termed rapid electrokinetic patterning (REP), is excellent in dynamic and programmable particle manipulation. Therefore, the detection could be complete in roughly 10 s. With an appropriate frequency modulation, the two DR biomarkers were detected at a time. The limit of detection (LOD) of the REP-enabled measurement reached as low as 100 pg/mL. The combined use of bead-based immunoassays and the optoelectrokinetic platform therefore provides an insightful measure to the early diagnosis of diseases.

Original languageEnglish
Title of host publicationSymposia
Subtitle of host publicationFluid Measurement and Instrumentation; Fluid Dynamics of Wind Energy; Renewable and Sustainable Energy Conversion; Energy and Process Engineering; Microfluidics and Nanofluidics; Development and Applications in Computational Fluid Dynamics; DNS/LES and Hybrid RANS/LES Methods
PublisherAmerican Society of Mechanical Engineers (ASME)
ISBN (Electronic)9780791858059
DOIs
Publication statusPublished - 2017 Jan 1
EventASME 2017 Fluids Engineering Division Summer Meeting, FEDSM 2017 - Waikoloa, United States
Duration: 2017 Jul 302017 Aug 3

Publication series

NameAmerican Society of Mechanical Engineers, Fluids Engineering Division (Publication) FEDSM
Volume1B-2017
ISSN (Print)0888-8116

Other

OtherASME 2017 Fluids Engineering Division Summer Meeting, FEDSM 2017
CountryUnited States
CityWaikoloa
Period17-07-3017-08-03

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

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