Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections

Wen Hsiang Su, Tien Yu Ho, Tsung Shan Tsou, Wen Ling Lee, Kuan Chin Wang, Yuan Yi Yu, Tien Jui Chen, Chia Hsuan Tan, Cheng Deng Kuo, Chien Sheng Chen, Peng Hui Wang

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Abstract

Objective: Cervicovaginitis is a highly prevalent disease that is a burden on healthcare globally. Immediate and adequate treatment can eradicate the infection and block subsequent complications. The feasibility of achip-based multiplexed immunoassay using liposomal nanovesicles was tested. Materials and Methods: A multiplexed immunoassay chip containing five antibodies for five pathogens (Chlamydia trachomatis, Escherichia coli, Neisseria gonorrhoeae, Streptococcus agalactiae, and Candida albicans) was established and tested. Four patients with spiking of candidiasis were enrolled. The difference between positive and negative readings was evaluated using the paired Student t test. Results: The detection threshold of Candida in this microarray was 100,000 CFU/mL in a vaginal sample, and the time required for the whole procedure was 3 hours. The testing of the four patients showed 100% for both sensitivity and specificity. Conclusion: This microarray chip was a rapid, easy, inexpensive and sensitive tool for detecting female lower genital tract Candida infection in a one-time vaginal sampling process, although the data on the four other pathogens were still unavailable. A larger population study is encouraged to test the validity of this multiplexed immunoassay chip.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalTaiwanese Journal of Obstetrics and Gynecology
Volume52
Issue number1
DOIs
Publication statusPublished - 2013 Mar 1

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All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

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