Development of an image-based algorithm for the motility characterizations of the nematode caenorhabditis elegans

Wan Jung Kuo, Han Sheng Chuang, Han Sheng Chuang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The nematode Caenorhabditis (C.) elegans has been widely used as a model animal for fundamental biological research. In order to investigate the effect of exercise on degenerative behaviors of C. elegans, such as physiological (lifespan, progeny) and biomechanical (propulsion, total power and swimming gait) properties, we developed a flow visualization technique to characterize the motility of C. elegans. Quantifying the motility of micro-organisms is always essential in understanding their biomechanical properties. Up to date, however, the direct measurement of the motility of C. elegans remains a big challenge due to lack of proper tools. Therefore, a simple image-based algorithm using a microparticle image velocimetry (μPIV) for deriving the kinetic power and propulsive force of the nematode C. elegans was developed in this study. For the measurement, each worm was confined in a 0.5 μL droplet which was sandwiched between two glass slides separated by two tapes. The motility of the confined worm was derived from the fluid motion according to the law of conservation of energy. The experimental result showed that our measured values appear to be in good agreements with the prior data. The image-based algorithm was proven to be a simple and automated measure for characterizing the dynamic motility of micro-swimmers. The study will eventually provide valuable information for treating and preventing degenerative diseases in higher animals.

Original languageEnglish
Title of host publication1st Global Conference on Biomedical Engineering and 9th Asian-Pacific Conference on Medical and Biological Engineering
EditorsFong-Chin Su, Ming-Long Yeh, Shyh-Hau Wang
PublisherSpringer Verlag
Pages107-110
Number of pages4
ISBN (Electronic)9783319122618
DOIs
Publication statusPublished - 2015
Event1st Global Conference on Biomedical Engineering, GCBME 2014 and 9th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2014 - Tainan, Taiwan
Duration: 2014 Oct 92014 Oct 12

Publication series

NameIFMBE Proceedings
Volume47
ISSN (Print)1680-0737
ISSN (Electronic)1433-9277

Other

Other1st Global Conference on Biomedical Engineering, GCBME 2014 and 9th Asian-Pacific Conference on Medical and Biological Engineering, APCMBE 2014
CountryTaiwan
CityTainan
Period14-10-0914-10-12

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Development of an image-based algorithm for the motility characterizations of the nematode caenorhabditis elegans'. Together they form a unique fingerprint.

  • Cite this

    Kuo, W. J., Chuang, H. S., & Chuang, H. S. (2015). Development of an image-based algorithm for the motility characterizations of the nematode caenorhabditis elegans. In F-C. Su, M-L. Yeh, & S-H. Wang (Eds.), 1st Global Conference on Biomedical Engineering and 9th Asian-Pacific Conference on Medical and Biological Engineering (pp. 107-110). (IFMBE Proceedings; Vol. 47). Springer Verlag. https://doi.org/10.1007/978-3-319-12262-5_30