Abstract
This study reports a new, simple and sensitive immunoassay that uses the impedance variation caused by silver nanoparticles (AgNPs) coated with an antibody. In this method, an electro-microchip is used to detect the immuno-reaction signal of antibody-antigen recognition, AgNPs as a label of antibody and a catalyst for silver precipitation, and the silver enhancement reaction is used to magnify the detection signal. Our model format is based on the sandwich immunoassay (three-layer format). The AgNPs were introduced into the electro-microchip by the specific binding of the antibodies and then coupled with silver enhancement solution to reduce silver ions to silver metal. The silver precipitation constructs a "bridge" between two electrodes of the electro-microchip allowing the electrons to pass. There was a significant difference in impedance between the experimental sample and the negative control after 10 min of reaction time. The proposed method requires less time and fewer steps than enzyme-linked immunosorbent assay (ELISA). It also has a high detection sensitivity (10 μg/mL of 1st antibody (IgG) immobilized on slides and 0.1 ng/mL of antigen (protein A)). Therefore, a new immunoassay is constructed using an electro-microchip, antibody-AgNPs conjugates, and a silver enhancement reaction.
| Original language | English |
|---|---|
| Pages (from-to) | 387-393 |
| Number of pages | 7 |
| Journal | Sensors and Actuators, B: Chemical |
| Volume | 139 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Jun 4 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry
Cite this
}
Development of an immunoassay based on impedance measurements utilizing an antibody-nanosilver probe, silver enhancement, and electro-microchip. / Yeh, Chia Hsien; Chen, Wei Ting; Lin, Hong-Ping; Chang, Tsung Chain; Lin, Yu-Cheng.
In: Sensors and Actuators, B: Chemical, Vol. 139, No. 2, 04.06.2009, p. 387-393.Research output: Contribution to journal › Article
TY - JOUR
T1 - Development of an immunoassay based on impedance measurements utilizing an antibody-nanosilver probe, silver enhancement, and electro-microchip
AU - Yeh, Chia Hsien
AU - Chen, Wei Ting
AU - Lin, Hong-Ping
AU - Chang, Tsung Chain
AU - Lin, Yu-Cheng
PY - 2009/6/4
Y1 - 2009/6/4
N2 - This study reports a new, simple and sensitive immunoassay that uses the impedance variation caused by silver nanoparticles (AgNPs) coated with an antibody. In this method, an electro-microchip is used to detect the immuno-reaction signal of antibody-antigen recognition, AgNPs as a label of antibody and a catalyst for silver precipitation, and the silver enhancement reaction is used to magnify the detection signal. Our model format is based on the sandwich immunoassay (three-layer format). The AgNPs were introduced into the electro-microchip by the specific binding of the antibodies and then coupled with silver enhancement solution to reduce silver ions to silver metal. The silver precipitation constructs a "bridge" between two electrodes of the electro-microchip allowing the electrons to pass. There was a significant difference in impedance between the experimental sample and the negative control after 10 min of reaction time. The proposed method requires less time and fewer steps than enzyme-linked immunosorbent assay (ELISA). It also has a high detection sensitivity (10 μg/mL of 1st antibody (IgG) immobilized on slides and 0.1 ng/mL of antigen (protein A)). Therefore, a new immunoassay is constructed using an electro-microchip, antibody-AgNPs conjugates, and a silver enhancement reaction.
AB - This study reports a new, simple and sensitive immunoassay that uses the impedance variation caused by silver nanoparticles (AgNPs) coated with an antibody. In this method, an electro-microchip is used to detect the immuno-reaction signal of antibody-antigen recognition, AgNPs as a label of antibody and a catalyst for silver precipitation, and the silver enhancement reaction is used to magnify the detection signal. Our model format is based on the sandwich immunoassay (three-layer format). The AgNPs were introduced into the electro-microchip by the specific binding of the antibodies and then coupled with silver enhancement solution to reduce silver ions to silver metal. The silver precipitation constructs a "bridge" between two electrodes of the electro-microchip allowing the electrons to pass. There was a significant difference in impedance between the experimental sample and the negative control after 10 min of reaction time. The proposed method requires less time and fewer steps than enzyme-linked immunosorbent assay (ELISA). It also has a high detection sensitivity (10 μg/mL of 1st antibody (IgG) immobilized on slides and 0.1 ng/mL of antigen (protein A)). Therefore, a new immunoassay is constructed using an electro-microchip, antibody-AgNPs conjugates, and a silver enhancement reaction.
UR - http://www.scopus.com/inward/record.url?scp=67349239806&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67349239806&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2009.03.029
DO - 10.1016/j.snb.2009.03.029
M3 - Article
VL - 139
SP - 387
EP - 393
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
IS - 2
ER -