Development of Non-contact Composite Temperature Sensing (CTS) for photothermal Real-time quantitative PCR Device

Hsin Yi Tsai, Liang Chieh Chao, Chih Ning Hsu, Kuo Cheng Huang, Yun Hao Tsai, Dar Bin Shieh, Chih Chung Yang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Real-time quantitative PCR device was emerging to be an essential platform in precision medicine and other genetic testing applications. The traditional heating method is extremely energy and time-consuming. Novel photothermal conversion based mechanism was reported recently that speed up the system to an unprecedented speed and energy efficiency and sensing of the temperature change became a challenging task. In general, the temperature of the reagent in the tubes was determined according to transform equations from the temperature of the heating block. However, the true temperature in each reagent was difficult to be accurately estimated. In this study, we proposed a new optical method to heat the reagent and integrated the thermocouple, metal tape, and thermal conducting rubber to serve as a composite temperature sensor (CTS) for measuring the radiant temperature from the tube. In addition, the temperature of the reagent was also detected by a thermocouple in the reagent. The relationship between the two temperature measurements was compared. The results showed that the CTS with copper tape was more sensitive and stable than that of other groups. A suitable dimension was determined to be 4 x 3 mm for the copper tape with a 1 mm thickness of the thermal conducting rubber. The mathematical correlation between the two measurements was evaluated and turned out to be 96.2 %. In the future, the CTS outside the tube could be applied to estimate the actual temperature of the reagent in the tube in further PCR applications.

Original languageEnglish
Title of host publication2020 IEEE Sensors Applications Symposium, SAS 2020 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728148427
DOIs
Publication statusPublished - 2020 Mar
Event15th IEEE Sensors Applications Symposium, SAS 2020 - Kuala Lumpur, Malaysia
Duration: 2020 Mar 92020 Mar 11

Publication series

Name2020 IEEE Sensors Applications Symposium, SAS 2020 - Proceedings

Conference

Conference15th IEEE Sensors Applications Symposium, SAS 2020
Country/TerritoryMalaysia
CityKuala Lumpur
Period20-03-0920-03-11

All Science Journal Classification (ASJC) codes

  • Computer Science Applications
  • Computer Vision and Pattern Recognition
  • Instrumentation

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