Development of protein chips based on self-assembled monolayer and protein A

Ling Sheng Jang, Hao Kai Keng, Yi Chu Hsu, Deirdre R. Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In order to improve the steric hindrances and less ordered structures in the long-chain SAMs, the SAM of 4,4-Dithiodibutyric acid (4,4- DTBA) was investigated to be a thin monolayer for the protein chip. This work presents the feasibility study of 4,4- DTBA as a monolayer of the protein chip based on gold surface. Characterizations of surface topography using atomic force microscopy (AFM), contact angle and fluorescence using protein A-FITC were performed to investigate the binding efficiency for the SAMs of 4,4- DTBA and 11- mercaptoundecanoic acid (11- MUA). The results show that the binding efficiency of 4,4- DTBA is higher than that of 11- MUA. The SAM of 4,4-DTBA can be used as a monolayer for the protein chip and may have a comparable binding efficiency.

Original languageEnglish
Title of host publicationISCAS 2006
Subtitle of host publication2006 IEEE International Symposium on Circuits and Systems, Proceedings
Pages4353-4358
Number of pages6
Publication statusPublished - 2006 Dec 1
EventISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems - Kos, Greece
Duration: 2006 May 212006 May 24

Publication series

NameProceedings - IEEE International Symposium on Circuits and Systems
ISSN (Print)0271-4310

Other

OtherISCAS 2006: 2006 IEEE International Symposium on Circuits and Systems
CountryGreece
CityKos
Period06-05-2106-05-24

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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